Anti-Mouse CXCL10

Purified Recombinant Mouse IP-10 (>98%)

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

Lyophilized

The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Next Day Ambient

Immunohistochemistry on Frozen Sections: This antibody should give satisfactory staining results when used on frozen sections at a concentration of 5-15 µg/ml.
Neutralization: This antibody is useful for neutralization of Mouse CXCL10 bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Mouse CXCL10 (at 0.5 ug/ml) is 5-25 µg/ml.

Goat Anti-Mouse Chemokine (C-X-C Motif) Ligand 10 (CXCL10) recognizes Mouse CXCL10. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Chemokine (C-X-C motif) ligand 10 (CXCL10) or IP-10 is a small cytokine belonging to the CXC chemokine family. CXCL10 is secreted by several cell types in response to IFN-γ. These cell types include monocytes, endothelial cells and fibroblasts.¹ CXCL10 has been attributed to several roles, such as chemoattraction for monocytes/macrophages, T cells, NK cells, and dendritic cells, promotion of T cell adhesion to endothelial cells, antitumor activity, and inhibition of bone marrow colony formation and angiogenesis.^2,3 CXCL10 plays a role in the regulation of tubular cell proliferation following renal ischemia-reperfusion injury.⁴

1. Luster, AD. et al.(1985) Nature 315: 672
2. Dufour, JH. et al.(2002) J. Immunol. 168
3. Angiolillo, AL. et al.(1995) J. Exp. Med. 182: 155
4. Kaneko, S. et al.(2008) Nephron Exp. Nephrol. 109:(1):c29-38