Anti-Mouse DPPIV/CD26

Anti-Mouse DPPIV/CD26

Product No.: D357


Clone 155202 Target Dipeptidyl Peptidase IV Formats AvailableView All Product Type Monoclonal Antibody Alternate Names CD26, DPP IV Isotype IgG2a Applications CyTOF® , ELISA Indirect , FC


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Antibody Details Product Details Reactive Species Mouse Host Species Rat Immunogen NS0-Derived Recombinant Mouse DPPIV Ectodomain Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2829937 Applications and Recommended Usage? Quality Tested by Leinco Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 - 1.0 µg/ml with a suitable second step reagent such as PN:R380. The detection sensitivity of this indirect ELISA for Mouse DPPIV is approximately 5 ng/well. Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing DPPIV. A suggested method would be to stain cells expressing DPPIV with 0.25 µg per 1 - 2.5 x 10⁵ cells in a total staining volume of ≤200 µl followed by PN:R485. Additional Applications Reported In Literature ? CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Specificity Rat Anti-Mouse Dipeptidyl Peptidase IV (DPPIV) (Clone 155202) recognizes an epitope on Mouse DPPIV. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. PubMed Dipeptidyl Peptidase IV NCBI Gene Bank ID 13482 UniProt.org More Information on Uniprot.org References & Citations View product citations for antibody PRODUCT_CODE on CiteAb Technical Protocols CyTOF® Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Mouse

Host Species

Rat

Immunogen

NS0-Derived Recombinant Mouse DPPIV Ectodomain

Endotoxin Level

<0.1 EU/µg as determined by the LAL method

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Storage and Handling

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2829937

Applications and Recommended Usage?
Quality Tested by Leinco

Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 - 1.0 µg/ml with a suitable second step reagent such as PN:R380. The detection sensitivity of this indirect ELISA for Mouse DPPIV is approximately 5 ng/well.
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing DPPIV. A suggested method would be to stain cells expressing DPPIV with 0.25 µg per 1 - 2.5 x 10⁵ cells in a total staining volume of ≤200 µl followed by PN:R485.

Additional Applications Reported In Literature ?

CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Rat Anti-Mouse Dipeptidyl Peptidase IV (DPPIV) (Clone 155202) recognizes an epitope on Mouse DPPIV. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

PubMed

Dipeptidyl Peptidase IV

NCBI Gene Bank ID

13482

UniProt.org

More Information on Uniprot.org