Anti-Mouse EDAR

Product No.: E271


Target EDAR Product Type Polyclonal Antibody Alternate Names Ectodysplasin A Receptor, DL, ED1R, ED3, ED5, EDA-A1R, EDA1R, EDA3, FLJ94390 Applications B , WB


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Antibody Details Product Details Reactive Species Mouse Host Species Goat Immunogen NS0-Derived Recombinant Mouse EDAR Extracellular Domain Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830043 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Mouse EDAR this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Mouse EDAR is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Additional Applications Reported In Literature ? Blocking: Approximately 1 - 4 μg/mL of this antibody will block 50% of 1 μg/mL of biotinylated rmEDA/his to immobilized rmEDAR/Fc chimera (100 μL of a 1 μg/mL solution was coated in each well) in a functional ELISA. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity Goat Anti-Mouse Ectodysplasin A Receptor (EDAR) recognizes Mouse EDAR. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Ectodysplasin A receptor, also known as EDAR is a cell surface receptor of the tumor necrosis factor receptor (TNFR) family involved in the development of hair follicles, teeth, and sweat glands. ¹ EDAR is the genetic determinant of hair thickness as well as a strong contributor to hair fiber thickness variation among Asian populations. ² PubMed EDAR NCBI Gene Bank ID 13608 UniProt.org More Information on Uniprot.org References & Citations 1. Headon, DJ. et al. (2008)Hum Genet. 29:1405 2. Tokunaga, K. et al. (2008)Hum Genet. 124:179 View product citations for antibody E271 on CiteAb Technical Protocols B Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Mouse

Host Species

Goat

Immunogen

NS0-Derived Recombinant Mouse EDAR Extracellular Domain

Endotoxin Level

<0.1 EU/µg as determined by the LAL method

Formulation

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

State of Matter

Lyophilized

Storage and Handling

The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2830043

Applications and Recommended Usage?
Quality Tested by Leinco

Western Blotting: To detect Mouse EDAR this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Mouse EDAR is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.

Additional Applications Reported In Literature ?

Blocking: Approximately 1 - 4 μg/mL of this antibody will block 50% of 1 μg/mL of biotinylated rmEDA/his to immobilized rmEDAR/Fc chimera (100 μL of a 1 μg/mL solution was coated in each well) in a functional ELISA.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Goat Anti-Mouse Ectodysplasin A Receptor (EDAR) recognizes Mouse EDAR. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background

Ectodysplasin A receptor, also known as EDAR is a cell surface receptor of the tumor necrosis factor receptor (TNFR) family involved in the development of hair follicles, teeth, and sweat glands. ¹
EDAR is the genetic determinant of hair thickness as well as a strong contributor to hair fiber thickness variation among Asian populations. ²

PubMed

EDAR

NCBI Gene Bank ID

13608

UniProt.org

More Information on Uniprot.org