Anti-Mouse FGF R2 (IIIc)

Product No.: F1082


Clone 133706 Target FGF R2 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names IIIc, Fibroblast Growth Factor Receptor 2 Isotype IgG2a Applications N , WB


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Antibody Details Product Details Reactive Species Mouse Host Species Rat Immunogen NS0 and Sf 21-Derived Extracellular Domains of Recombinant Mouse FGF Receptor 2 Isoforms Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830128 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Mouse FGF R2 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse FGF R2 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Additional Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Mouse FGF R2 bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Mouse FGF R2 (at 3 ng/ml) is 0.1 - 0.4 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Specificity Rat Anti-Mouse Fibroblast Growth Factor Receptor 2 (FGF R2) (Clone 133706) recognizes an epitope on Mouse FGF R2. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody displays 100% cross-reactivity with IIIc isoforms of rhFGF R2 and no cross-reactivity with IIIb isoforms of rhFGF R2 or any isoforms of rhFGF R1, rmFGF R3 or rhFGF R4. PubMed FGF R2 NCBI Gene Bank ID 14183 UniProt.org Information on Uniprot.org References & Citations View product citations for antibody PRODUCT_CODE on CiteAb Technical Protocols N Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Mouse

Host Species

Rat

Immunogen

NS0 and Sf 21-Derived Extracellular Domains of Recombinant Mouse FGF Receptor 2 Isoforms

Endotoxin Level

<0.1 EU/µg as determined by the LAL method

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Storage and Handling

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2830128

Applications and Recommended Usage?
Quality Tested by Leinco

Western Blotting: To detect Mouse FGF R2 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse FGF R2 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.

Additional Applications Reported In Literature ?

Neutralization: This antibody is useful for neutralization of Mouse FGF R2 bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Mouse FGF R2 (at 3 ng/ml) is 0.1 - 0.4 µg/ml.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Rat Anti-Mouse Fibroblast Growth Factor Receptor 2 (FGF R2) (Clone 133706) recognizes an epitope on Mouse FGF R2. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody displays 100% cross-reactivity with IIIc isoforms of rhFGF R2 and no cross-reactivity with IIIb isoforms of rhFGF R2 or any isoforms of rhFGF R1, rmFGF R3 or rhFGF R4.

PubMed

FGF R2

NCBI Gene Bank ID

14183

UniProt.org

Information on Uniprot.org