Anti-Mouse FGF R3

NS0-Derived Recombinant Mouse FGF R3

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

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Neutralization: The peptide may be used to neutralize the binding activity of PN:T361 by incubation of an equal volume of the peptide and antibody for 30 minutes at 37°C for Western blotting experiments.

Rat Anti-Mouse Fibroblast Growth Factor Receptor 3 (FGF R3) (Clone 136812) recognizes an epitope on Mouse FGF R3. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Fibroblast growth factor receptor 3 is receptor tyrosine kinases expressed on most types of cells.¹ FGFR3 protein plays a role in the development and maintenance of bone and brain tissue and has several important cellular functions such as regulation of cell growth and division, determination of cell type, formation of blood vessels, wound healing, and embryo development.² FGFR3 result in autosomal dominant human skeletal dysplasias and is a major cause of benign epidermal tumors in humans.³

1. Hicks, D. et al. (1999) J Mol Neurosci. 13: 187
2. Hartmann, A. et al. (2006) J Clin Invest. 116(8):2201-2207.
3. Radvanyi, F. et al. (2005) Human Molecular Genetics 14: 1153