Anti-Mouse Flt-3 - Biotin - Leinco Technologies

Anti-Mouse Flt-3 – Biotin

Product No.: F1100


Target Flt-3 Product Type Polyclonal Antibody Alternate Names FMS-Like Tyrosine Kinase 3, FLT3, STK1, Cluster of Differentiation 135 (CD135), Fetal Liver Kinase-2 (FLK2) Applications FC , WB


Select Product Size 50 µg — $570.00	Qty Max: Min: 1 Step: 1 $570.00 ADD TO CART Login For mAb Advantage Pricing Contact Us for Bulk Quantities


Antibody Details Product Details Reactive Species Mouse Host Species Goat Immunogen Purified Recombinant Mouse Flt-3 (Accession # Q00342) Formulation This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830147 Applications and Recommended Usage? Quality Tested by Leinco Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing Flt-3. A suggested method would be to stain cells expressing Flt-3 with 3 - 10 µg per 1 x 10⁶ cells in a total staining volume of 100 µl followed by PN:A104. Western Blotting: To detect Mouse Flt-3 this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Mouse Flt-3 is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:F1054. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity Goat Anti-Mouse FMS-Like Tyrosine Kinase 3 (Flt-3) recognizes Mouse Flt-3. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Fms-Like tyrosine kinase 3 (FLT3) is a member of the class III tyrosine kinase receptor family, normally expressed in hematopoietic, immune and neural systems.¹ FLT3 is expressed in various lymphohematopoietic cells and tissues, including a series of immature cell lines and leukemias of lymphocytic origin.² FLT3 is a promising target for the therapeutic intervention for acute leukemias, particularly acute myeloid leukemia (AML) which are severe and aggressive malignancy.¹ PubMed Flt-3 NCBI Gene Bank ID 14255 UniProt.org More Information on Uniprot.org References & Citations 1. Botta, M. et al. (2008) Curr Med Chem. 15: 3113 2. Birnbaum, D. et al. (1993) Blood 82: 1110 View product citations for antibody PRODUCT_CODE on CiteAb Technical Protocols Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Mouse

Host Species

Goat

Immunogen

Purified Recombinant Mouse Flt-3 (Accession # Q00342)

Formulation

This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.

State of Matter

Lyophilized

Storage and Handling

The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2830147

Applications and Recommended Usage?
Quality Tested by Leinco

Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing Flt-3. A suggested method would be to stain cells expressing Flt-3 with 3 - 10 µg per 1 x 10⁶ cells in a total staining volume of 100 µl followed by PN:A104.
Western Blotting: To detect Mouse Flt-3 this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Mouse Flt-3 is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:F1054.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Goat Anti-Mouse FMS-Like Tyrosine Kinase 3 (Flt-3) recognizes Mouse Flt-3. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background

Fms-Like tyrosine kinase 3 (FLT3) is a member of the class III tyrosine kinase receptor family, normally expressed in hematopoietic, immune and neural systems.¹ FLT3 is expressed in various lymphohematopoietic cells and tissues, including a series of immature cell lines and leukemias of lymphocytic origin.² FLT3 is a promising target for the therapeutic intervention for acute leukemias, particularly acute myeloid leukemia (AML) which are severe and aggressive malignancy.¹

PubMed

Flt-3

NCBI Gene Bank ID

14255

UniProt.org

More Information on Uniprot.org