Anti-Mouse GDF-5 - Biotin - Leinco Technologies

Anti-Mouse GDF-5 – Biotin

Product No.: G696


Target GDF-5 Product Type Polyclonal Antibody Alternate Names Growth Differentiation Factor 5, CDMP1, LAP4, SYNS2 Applications IHC FFPE , WB


Select Product Size 50 µg — $550.00	Qty Max: Min: 1 Step: 1 $550.00 ADD TO CART Login For mAb Advantage Pricing Contact Us for Bulk Quantities


Antibody Details Product Details Reactive Species Mouse Host Species Goat Immunogen Purified Recombinant Mouse GDF-5 (Accession # P43027) Formulation This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830285 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Mouse GDF-5 this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Mouse GDF-5 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:G654. Additional Applications Reported In Literature ? IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 5-15 µg/ml. The recommended secondary antibody for IHC is PN:A106. For chromogenic detection with high signal and low background use PN:D100 or PN:K107. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity Goat Anti-Mouse Growth Differentiation Factor 5 (GDF-5) recognizes Mouse GDF-5. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Growth differentiation factor 5 (cartilage-derived morphogenetic protein-1), also known as GDF5 is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. GDF5 is expressed in the developing central nervous system.¹ It increases the survival of neurones that respond to a neurotransmitter called dopamine, and is a potential therapeutic molecule associated with Parkinson's disease.² PubMed GDF-5 NCBI Gene Bank ID 14563 UniProt.org More Information on Uniprot.org References & Citations 1. Sullivan, A. et al. (2004) J Neurocytol 33: 479 2. O'Keeffe, G. et al. (2005) J Anat 207: 219 View product citations for antibody PRODUCT_CODE on CiteAb Technical Protocols IHC FFPE Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Mouse

Host Species

Goat

Immunogen

Purified Recombinant Mouse GDF-5 (Accession # P43027)

Formulation

This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.

State of Matter

Lyophilized

Storage and Handling

The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2830285

Applications and Recommended Usage?
Quality Tested by Leinco

Western Blotting: To detect Mouse GDF-5 this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Mouse GDF-5 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:G654.

Additional Applications Reported In Literature ?

IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 5-15 µg/ml. The recommended secondary antibody for IHC is PN:A106. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Goat Anti-Mouse Growth Differentiation Factor 5 (GDF-5) recognizes Mouse GDF-5. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background

Growth differentiation factor 5 (cartilage-derived morphogenetic protein-1), also known as GDF5 is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. GDF5 is expressed in the developing central nervous system.¹ It increases the survival of neurones that respond to a neurotransmitter called dopamine, and is a potential therapeutic molecule associated with Parkinson's disease.²

PubMed

GDF-5

NCBI Gene Bank ID

14563

UniProt.org

More Information on Uniprot.org