Anti-Mouse H-2Kd – Purified

Anti-Mouse H-2Kd – Purified

Product No.: H142

[product_table name="All Top" skus="H142"]

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Clone
A4C8.1-Do9
Target
I H-2Kd
Formats AvailableView All
Product Type
Monoclonal Antibody
Isotype
Mouse IgG2b κ
Applications
FC
,
IHC FF
,
IP
,
WB

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Antibody Details

Product Details

Reactive Species
Class
Host Species
Mouse
Product Concentration
0.5 mg/ml
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This antibody is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for clone A4C8.1-Do9 antibody for staining cells in flow cytometry is ≤ .25 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
Additional Applications Reported In Literature ?
IP
WB
IHC FF
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Class I H-2Kd (Clone Do9) recognizes Class I H-2Kd. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution
Classical class I; ubiquitously distributed.
PubMed
Research Area
Immunology
.
Innate Immunity

References & Citations

Hauptfeld, V. (1984) Immunogenetics 19:169
Flow Cytometry
IHC FF
Immunoprecipitation Protocol
General Western Blot Protocol

Certificate of Analysis

Formats Available

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.