Anti Mouse/Human IL-1ra/IL-1F3

Anti Mouse/Human IL-1ra/IL-1F3

Product No.: I-668

[product_table name="All Top" skus="I-668"]

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Clone
40007
Target
IL-1ra/IL-1F3
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Interleukin-1 Receptor Antagonist
Isotype
IgG2a
Applications
WB

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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Rat
Immunogen
E. coli-Derived Recombinant Mouse Interleukin-1 Receptor a (Accession # P25085)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Mouse IL-1ra this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse IL-1ra is 20 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rat Anti-Mouse Interleukin 1 Receptor Antagonist (IL-1ra) (Clone 40007) recognizes an epitope on Mouse IL-1ra. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

References & Citations

General Western Blot Protocol

Certificate of Analysis

Formats Available

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Prod No.
Description
I-668
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.