Anti-Mouse I-A/I-E (MHC Class II) – Purified (PhenoCyler-Fusion (CODEX)® Ready)

Clone M5/114.15.2 has been validated for use on CODEX® using mouse spleen fresh frozen (FF) tissue.

C57BL/6 Spleen Cells

This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4.

This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

AB_2893767

Clone M5/114.15.2 recognizes an epitope on the mouse MHC class II molecules I-A^b, I-A^d, I-A^q, I-E^d, and I-E^k. Clone M5/114.15.2 does not react with I-A^f, I-A^k, or I-A^s.

I-A^b/d/q and I-E^d/k are expressed on antigen-presenting cells, including macrophages, monocytes, DCs, and B cells, and activated T cells from mice of the I-A^b/d/q and I-E^d/k haplotypes.

MHC class II antibody, clone M5/114.15.2, recognizes the major histocompatibility complex (MHC) class II molecules I-A^b, I-A^d, I-A^q, I-E^d, and I-E^k. MHC class II is constitutively expressed on professional antigen-presenting cells (APCs), including macrophages/monocytes, dendritic cells (DCs), and B cells, and is induced on T cells upon activation¹. MHC class II consists of two transmembrane proteins, a 35 kDa α (heavy) chain and 29 kDa β (light) chain². The N-terminal α1 and β1 domains form the antigen-binding groove, which binds 13-25 aa peptides derived from exogenous antigens³. On APCs, MHC class II plays a critical role in the adaptive immune response by presenting phagocytosed antigens to helper CD4 T cells. The T cell receptor (TCR)/CD3 complex of CD4 T cells interacts with peptide-MHC class II, which induces CD4 T cell activation leading to the coordination and regulation of other effector cells. CD4 molecules also bind to MHC class II, which helps augment TCR signaling⁴. It has also been demonstrated that MHC class II express on activated T cells are capable of antigen presentation⁵ and can transduce signals into T cells, enhancing T cell proliferation and activity⁶.

CD3/TCR, CD4

1. Holling TM, et al. (2004) Hum Immunol. 65(4):282-90
2. Mitaksov V & Fremont DH. (2006) J Biol Chem. 281(15):10618-25
3. Wieczorek M, et al. (2017) Front Immunol. 8:292
4. Artyomov MN, et al. (2010) Proc Natl Acad Sci USA. 107(39):16916-16921
5. Barnaba V, et al. (1994) Eur J Immunol. 24(1):71-5
6. Di Rosa F, et al. (1993) Hum Immunol. 38(4):251-60