Anti-Mouse ICAM-1

NS0-Derived Recombinant Mouse ICAM-1 Extracellular Domain

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

Lyophilized

The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Next Day Ambient

Blocking: This antibody shows inhibition in the HSB2 adhesionassay. The adhesion of PMA treated human HSB2 cells (5 x 104 cells/well) to12.5 μg/mL immobilized rmICAM-1/Fc (100 μL/well) was maximally inhibited(80 - 100%) following pre-treatment with antibody for 1 hour using 100 μL of a5 μg/mL antibody solution per well.
Immunohistochemistry: Suitable for use at concentration of 5-15 µg/mL.

Goat Anti-Mouse Intercellular Adhesion Molecule-1 (ICAM-1) recognizes Mouse ICAM-1. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

ICAM-1 is a 55 kDa glycoprotein that is part of the Ig superfamily. It is heavily glycosylated to form 75 kDa to 115 kDa. ICAM-1 is known to be an adhesion and viral entry molecule, and its long suspected involevement in signal transduction is being elucidated. The signal-transducing functions of ICAM-1 appear to be mainly associated with proinflammatory pathways. Furthermore, ICAM-1 signaling appears to act as a beacon for inflammatory immune cells such as macrophages and granulocytes bringing about inflammation via lymphocyte trafficking. ICAM-1 is essential for the transmigration of leukocytes out of blood vessels and into tissues, and is a marker of endothelial dysfunction leading to damaging vascular disorders in umbilical and placental vascular tissue of gestational pregnancies. ICAM-1 is the receptor for rhinoviruses (the cause of most common colds) and malaria, and plays an inflammatory role in ocular allergies.

CD54 is present on endothelial cells, lymphocytes, epithelial cells, dendritic cells and keratinocytes.

1. Li, S. et al. (2009) Biochem Biophys Res Commun. 381: 459
2. Wolf, S. et al. (2009) Pharmacol Rep 61: 22
3. Ozcan, U et al. (2009) Arch Gynecol Obstet.