Anti-Mouse ICAM-1 (CD54) (Clone BE29G1) – Purified in vivo GOLD™ Functional Grade

Purified Recombinant Mouse ICAM-1 (>98%)

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.

Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Next Day 2-8°C

Rat Anti-Mouse CD54 (Clone BE29G1) recognizes the (Mr 95 kD) cell surface glycoprotein known as intracellular adhesion molecule ICAM-1. ICAM-1 is the receptor for LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18).^1,2,3

Intercellular adhesion molecule-1 (ICAM-1) is one of an important cell adhesion molecules (CAMs) family transmembrane glycoprotein¹ and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines.² ICAM-1 is a marker of endothelial dysfunction leading to damaging vascular disorders, in umbilical and placental vascular tissue of gestational pregnancies.³

The CD54 antigen is present on endothelial cells, lymphocytes, epithelial cells, dendritic cells and keratinocytes. It has been demonstrated that ICAM-1 enhances T-cell activation.

CD11a/CD18 (LFA-1) or CD11b/CD18 (Mac-1) and CD11c/CD18, CD43, hyaluronan, fibrinogen

1. Li S et al. (2009) Biochem Biophys Res Commun. 381: 459
2. Wolf S et al. (2009) Pharmacol Rep 61: 22
3. Ozcan U et al. (2009) Arch Gynecol Obstet.