Anti-Mouse IFN-γ

E. coli-Derived Recombinant Mouse IFN-γ

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Next Day Ambient

N This antibody is useful for neutralization of Mouse IFN-γ bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Mouse IFN-γ (at 2.5 ng/ml) is 0.075 - 0.3 µg/ml.
CyTOF® Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Rat Anti-Mouse Interferon Gamma (IFN-γ) (Clone 37895) recognizes an epitope on Mouse IFN-γ. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody does not cross-react with rhIFN-γ, rrIFN-γ, rpIFN-γ, rrmIFN-γ, rfeIFN-γ or rcaIFN-γ.

Mouse IFN-gamma is an acid-labile interferon produced by CD4 and CD8 T lymphocytes as well as activated NK cells. IFN-gamma receptors are present in most immune cells, which respond to IFN-gamma signaling by increasing the surface expression of class I MHC proteins. This promotes the presentation of antigen to T-helper (CD4+) cells. IFN-gamma signaling in antigen-presenting cells and antigen-recognizing B and T lymphocytes regulates the antigen-specific phases of the immune response. Additionally, IFN-gamma stimulates a number of lymphoid cell functions including the anti-microbial and anti-tumor responses of macrophages, NK cells, and neutrophils. Human IFN-gamma species-specific and is biologically active only in human and primate cells.