Anti-Mouse IFN-γ (Clone R4-6A2) – Biotin

Anti-Mouse IFN-γ (Clone R4-6A2) – Biotin

Product No.: I-1132

[product_table name="All Top" skus="I-1132"]

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Clone
R4-6A2
Target
IFN-γ
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Immune Interferon, Type II Interferon, T Cell Interferon, MAF, IFNG, IFG, IFI
Isotype
Rat IgG1 κ
Applications
ELISA Cap
,
ELISPOT
,
IHC FF
,
N

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Select Product Size
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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Rat
Immunogen
Purified Mouse IFN-γ
Product Concentration
0.5 mg/ml
Formulation
This biotinylated antibody is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This biotinylated antibody is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA Cap R4-6A2 antibody is useful as a detection antibody for a sandwich ELISA with a concentration range of 0.5-2.0 µg/ml as recommended.
Additional Applications Reported In Literature ?
ELISPOT Cap
ELISPOT Det
N
Additional Reported Applications For Relevant Conjugates ?
ELISPOT Cap
ELISPOT Det
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Description

Specificity
Rat Anti-Mouse Interferon Gamma (IFN-γ) (Clone R4-6A2) recognizes an epitope on Mouse IFN-γ. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
Interferon-gamma (IFN-γ) or type II interferon is a dimerized soluble cytokine that is the only member of the type II class of interferons.1 It is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. IFNG is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops.2 IFN-γ has antiviral, immunoregulatory, and anti-tumour properties.3
PubMed
NCBI Gene Bank ID
Research Area
Cell Biology
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Immunology
.
Neuroinflammation
.
Neuroscience
.
Other Molecules

References & Citations

1. Goeddel DV et al. (1982) Nature 298: 859
2. Wilson CB et al. (2007) Adv. Immunol. 96: 41
3. Hume DA et al. (2004) J Leukoc Biol. 75: 163
Elisa Sandwich Protocol
ELISPOT
IHC FF
N

Certificate of Analysis

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.