Anti-Mouse IFNγ (Clone XMG1.2) – Purified in vivo GOLD™ Functional Grade

Anti-Mouse IFNγ (Clone XMG1.2) – Purified in vivo GOLD™ Functional Grade

Product No.: I-1119

[product_table name="All Top" skus="I-1119"]

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Clone
XMG1.2
Target
IFN-γ
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Immune Interferon, Type II Interferon, T Cell Interferon, MAF, IFNG, IFG, IFI
Isotype
Rat IgG1 κ
Applications
CyTOF®
,
ELISA Cap
,
ELISPOT
,
ICFC
,
IHC FF
,
in vivo
,
N
,
WB

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Select Product Size

Data

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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Rat
Recommended Isotype Controls
Recommended Dilution Buffer
Immunogen
Purified Recombinant Mouse IFN-γ (>98%)
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
< 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA Cap XMG1.2 antibody is useful as a detection antibody for a sandwich ELISA with a concentration range of 0.5-2.0 µg/ml as recommended.
WB The suggested concentration for this XMG1.2 antibody for use in western blotting is 1-10 μg/ml.
Additional Applications Reported In Literature ?
ICFC
ELISPOT Cap
ELISPOT Det
CyTOF®
N
Additional Reported Applications For Relevant Conjugates ?
IHC FF
WB

For specific conjugates of this clone, review literature for suggested application details.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Description

Specificity
Rat Anti-Mouse Interferon Gamma (IFN-γ) (Clone XMG1.2) recognizes an epitope on Mouse IFN-γ. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
Interferon-gamma (IFN-γ) or type II interferon is a dimerized soluble cytokine that is the only member of the type II class of interferons.1 It is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. IFNG is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops.2 IFN-γ has antiviral, immunoregulatory, and anti-tumour properties.3
PubMed
NCBI Gene Bank ID
Research Area
Cell Biology
.
Immunology
.
Neuroinflammation
.
Neuroscience
.
Other Molecules

References & Citations

1). Goeddel DV et al. (1982) Nature 298: 859
2.) Wilson CB et al. (2007) Adv. Immunol. 96: 41
3.) Hume DA et al. (2004) J Leukoc Biol. 75: 163
4.) Teijaro et al. (2020) bioRxiv 22: 24
5.) Hawman DW, et al. (2021) Microorganisms 9(2):279 Journal Link
CyTOF®
Elisa Sandwich Protocol
ELISPOT
ICFC
IHC FF
in vivo Protocol
N
General Western Blot Protocol

Certificate of Analysis

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.