Anti-Mouse IFNß – Purified in vivo GOLD™ Functional Grade
Anti-Mouse IFNß – Purified in vivo GOLD™ Functional Grade
Product No.: I-1182
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Clone HDß-4A7 Target IFNβ Formats AvailableView All Product Type Monoclonal Antibody Alternate Names IFN-b, IFN-beta, Type-1 interferon, Clone HDb-4A7 Isotype Mouse IgG2a Applications B , FA , in vivo , N , WB |
Data
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Mouse Recommended Isotype Controls Recommended Dilution Buffer Immunogen Plasmid DNA encoding murine IFNb Product Concentration ≥ 5.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2737532 Additional Applications Reported In Literature ? FA B N WB In Vitro Activity of HDß-4A7 - Inhibits IFNb-induced Stat1 phosphorylation (level of inhibition is similar to that seen using anti-IFNAR1 mAb (MAR1-5A3). Blocks IFNb induction of MHC-I expression (H2-Kb) using L929 or fibrosarcoma cell lines. Neutralizes IFNb-induced antiviral activity in vitro following infection of L929 cells with VSV. In Vivo Activity of HDß-4A7 circulates with a half-life of ~ 14 days. Blocks in vivo IFNb leading to increased the lethality of mice infected with West Nile Virus (WNV), similar to susceptibility of IFNb-/- mice. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Mouse Anti-Mouse Interferon-beta (IFN-ß) (Clone HDß-4A7) recognizes an epitope on Mouse IFN-ß and binds specifically to murine IFNß and does not bind murine IFNg or several different IFNa species (a-1,4,5 or 13); HDb-4A7 binds both recombinant and natural forms. This antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. NCBI Gene Bank ID UniProt.org Research Area Apoptosis . Cell Biology . Cell Death . Signal Transduction . Tumor Suppressors References & Citations1. Oldstone, Michael B. A. et al. (2017) Proc Natl Acad Sci U S A. 114(14): 3708–3713. PubMed Technical ProtocolsB FA  N  Certificate of Analysis |
Formats Available
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Prod No. | Description |
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I-1182 | |
B659 |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
