Anti-Mouse IL-17D (Clone 312724)

Product No.: I-880


Clone 312724 Target IL-17D Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Interleukin-17D Isotype IgG2a Applications CyTOF® , FC , WB


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Data


Antibody Details Product Details Reactive Species Mouse Host Species Mouse Immunogen Purified Recombinant Mouse IL-17D (>98%) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830965 Applications and Recommended Usage? Quality Tested by Leinco Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing IL-17D. A suggested method would be to stain cells expressing IL-17D with 2.5 µg/10⁶ cells in a total staining volume of 200 µl followed by PN:M1189. Western Blotting: To detect Mouse IL-17D this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Mouse IL-17D is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:I-332. Additional Applications Reported In Literature ? CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Specificity Mouse Anti-Mouse Interleukin 17D (IL-17D) (Clone 312724) recognizes an epitope on Mouse IL-17D. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. PubMed IL-17D NCBI Gene Bank ID 239114 UniProt.org Information on Uniprot.org References & Citations View product citations for antibody PRODUCT_CODE on CiteAb Technical Protocols CyTOF® Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Mouse

Host Species

Mouse

Immunogen

Purified Recombinant Mouse IL-17D (>98%)

Endotoxin Level

<0.1 EU/µg as determined by the LAL method

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2830965

Applications and Recommended Usage?
Quality Tested by Leinco

Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing IL-17D. A suggested method would be to stain cells expressing IL-17D with 2.5 µg/10⁶ cells in a total staining volume of 200 µl followed by PN:M1189.
Western Blotting: To detect Mouse IL-17D this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Mouse IL-17D is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:I-332.

Additional Applications Reported In Literature ?

CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Mouse Anti-Mouse Interleukin 17D (IL-17D) (Clone 312724) recognizes an epitope on Mouse IL-17D. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

PubMed

IL-17D

NCBI Gene Bank ID

239114

UniProt.org

Information on Uniprot.org