Anti-Mouse IL-21R (Clone 4A9) – PE

Rat YB2/0 hybridoma transfectant cell line expressing truncated IL-21R

This R-phycoerythrin (R-PE) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

liquid

Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

This R-phycoerythrin (R-PE) conjugate is stable when stored at 2-8°C. Do not freeze.

Research Use Only

2-8°C Wet Ice

Blue Laser (488 nm) and/or Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Clone 4A9 activity is directed against murine Interleukin-21 Receptor (CD360).

Interleukin-21 receptor (IL-21R) is a single pass type I membrane protein and a member of the type I cytokine receptor family. It is the receptor for Interleukin-21 (IL-21) and upon binding forms a heterodimer with the common gamma subunit (CD132; IL-2RG). IL-21R and IL-21 are important for the proliferation and differentiation of B cells, T cells, and natural killer cells. They function in survival signalling for antiviral CD8⁺ T cells during chronic infections and induce growth and differentiation of mature B cells in Ig-secreting plasma cells. Additionally, they prevent T cell exhaustion and viral persistence by limiting virus driven Treg cell proliferation¹. IL-21 and IL21-R function as early as the pro-B cell stage, with receptor-mediated signalling increasing as cells mature². When IL-21R binds IL-21, the JAK/STAT transduction pathway is activated.

4A9 was generated by immunizing Lewis rats with IL-21R-transfected YB2/0 and subsequent fusion of immunized spleen cells to SP2/0³. FACS analysis and immunoprecipitation studies show that 4A9 selectively binds to IL-21R. Additionally, 4A9 stains the majority of spleen cells from wild type C57BL/6 mice but not IL-21R deficient mice. IL-21 blocks the binding of 4A9 to IL-21R-transfected YB2/0 cells.

IL-21R is expressed on most B cells as well as some T cells and NK cells.

IL-21

1. Schmitz I, Schneider C, Fröhlich A, et al. PLoS Pathog. 9(5):e1003362. 2013.
2. Simard N, Konforte D, Tran AH, et al. J Immunol. 186(9):5244-5253. 2011.
3. Jin H, Carrio R, Yu A, et al. J Immunol. 173(1):657-665. 2004.
4. DeKoter RP, Geadah M, Khoosal S, et al. J Immunol. 185(12):7374-7384. 2010.