Anti-Mouse IL-3 Rβ

Anti-Mouse IL-3 Rβ

Product No.: I-724


Target IL-3 R beta Product Type Polyclonal Antibody Alternate Names Interleukin-3 Receptor Beta, AIC2A, CD131 Applications CyTOF® , FC , N , WB


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Antibody Details Product Details Reactive Species Mouse Host Species Goat Immunogen NS0 - Derived Reombinant Mouse Interleukin-3 Receptor β Extracellular Domain Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2830845 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Mouse IL-3 Rβ this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Mouse IL-3 Rβ is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Flow Cytometry: Suitable for use at concentration of 0.25 µg/10⁶ cells. Additional Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Mouse IL-3 Rβ bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Mouse IL-3 Rβ (at 0.5 ng/ml) is 5 - 15 µg/ml. CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Specificity Goat Anti-Mouse Interleukin 3 Receptor Beta (IL-3 Rβ) recognizes Mouse IL-3 Rβ. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. PubMed IL-3 R beta NCBI Gene Bank ID 12984 UniProt.org More Information on Uniprot.org References & Citations View product citations for antibody PRODUCT_CODE on CiteAb Technical Protocols CyTOF® N Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Mouse

Host Species

Goat

Immunogen

NS0 - Derived Reombinant Mouse Interleukin-3 Receptor β Extracellular Domain

Endotoxin Level

<0.1 EU/µg as determined by the LAL method

Formulation

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

State of Matter

Lyophilized

Storage and Handling

The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day 2-8°C

RRIDAB_2830845

Applications and Recommended Usage?
Quality Tested by Leinco

Western Blotting: To detect Mouse IL-3 Rβ this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Mouse IL-3 Rβ is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Flow Cytometry: Suitable for use at concentration of 0.25 µg/10⁶ cells.

Additional Applications Reported In Literature ?

Neutralization: This antibody is useful for neutralization of Mouse IL-3 Rβ bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Mouse IL-3 Rβ (at 0.5 ng/ml) is 5 - 15 µg/ml.
CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Goat Anti-Mouse Interleukin 3 Receptor Beta (IL-3 Rβ) recognizes Mouse IL-3 Rβ. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

PubMed

IL-3 R beta

NCBI Gene Bank ID

12984

UniProt.org

More Information on Uniprot.org