Anti-Mouse IL-4 (Clone 11B11) – Purified in vivo GOLD™ Functional Grade
Anti-Mouse IL-4 (Clone 11B11) – Purified in vivo GOLD™ Functional Grade
Product No.: I-1071
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Clone 11B11 Target IL-4 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Interleukin-4, BCGF, BCDF, B Cell Stimulating Factor, BSF-1, IaIF, HCGF, MCGF2, MFF, TCGF-2 Isotype Rat IgG1 κ Applications CyTOF® , ELISA Cap , ELISPOT , FA , ICC , IHC , in vivo , IP , N |
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Recommended Isotype Controls Recommended Dilution Buffer Immunogen Partially Purified Native Mouse IL-4 Product Concentration ≥ 5.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2830405 Applications and Recommended Usage? Quality Tested by Leinco ELISAThis antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 0.5-2.0 μg/ml. Titration of the reagent is recommended for optimal performance for each application. Additional Applications Reported In Literature ? N IHC ICC IP CyTOF® FA Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone 11B11 recognizes an epitope on mouse IL-4. Background IL-4 antibody, 11B11, recognizes mouse IL-4, also known as B-cell differentiation factor (BCDF) and B-cell stimulatory factor (BSF1). IL-4 is a pleiotropic 20 kDa cytokine secreted by T helper 2 cells (Th2) cells, natural killer T (NKT) cells, mast cells, eosinophils, and basophils1,2. IL-4 signaling through the IL-4 receptor alpha chain (IL-4Ra) induces the differentiation of naive T cells to Th2 cells3,4 and stimulates B cell differentiation, proliferation, and class switching to IgG1 and IgE isotypes5. In addition, IL-4 is implicated in normal wound healing6,7 and airway inflammation in patients with asthma8. Antigen Distribution IL-4 is secreted by Th2 cells, NKT cells, mast cells, eosinophils, and basophils. Matched Pair Clone 11B11 can be used as the capture antibody in a sandwich ELISA or ELISPOT, using biotinylated clone BVD6-24G2 for detection. PubMed NCBI Gene Bank ID UniProt.org Research Area Immunology . Other Molecules References & Citations1. Yoshimoto T & Paul WE. (1994) J Exp Med. 179(4):1285–95 2. Kipnis J., at al. (2012) J Immunol. 189(9):4213-4219 3. Groth B., et al. (1992) J Exp Med. 176:1091–8 4. Murphy KM., et al. (1992) Proc Natl Acad Sci USA. 89:6065–9 5. Paul WE., et al. (1999) Annual Rev Immunol. 17:701-38 6. Maquart FX., et al. (2000) Lab Invest. 80(8):1337-43 7. Godeau, G., et al. (2000) Lab Invest. 80,1337–1343 8. Steinke JW & Borish L. (2001) Respir Res. 2(2):66-70 Technical ProtocolsCyTOF®  ELISPOT FA ICC    N Certificate of Analysis |
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I-1071 | |
I-1043 | |
I-1080 | |
I-1075 | |
I-1077 | |
I-1074 | |
I-2070 |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
