Anti-Mouse IL-4 R (Clone 129801)
Anti-Mouse IL-4 R (Clone 129801)
Product No.: I-676
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Clone 129801 Target IL-4 R Formats AvailableView All Product Type Monoclonal Antibody Alternate Names CD124, Interleukin-4 Receptor Isotype IgG2b Applications WB |
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Immunogen NS0-Derived Recombinant Mouse Interleukin-4 Receptor Extracellular Domain Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830797 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Mouse IL-4 R this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse IL-4 R is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Rat Anti-Mouse Interleukin 4 Receptor (IL-4 R) (Clone 129801) recognizes an epitope on Mouse IL-4 R. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody shows less than 1% cross-reactivity with rhIL-4 R. Background IL-4 can signal through type I and type II receptor complexes, which share a common gamma chain. The type I receptor contains in addition to the gamma chain an IL-4R alpha subunit, whereas the type II receptor contains the IL-13R alpha. The secreted extracellular domain of IL-4R alpha, called sIL-4R alpha, binds IL-4 and antagonizes its activity. It plays an important role in regulating the differentiation of naive CD4+ T cells and class switching to IgG1 and IgE. PubMed NCBI Gene Bank ID UniProt.org References & CitationsTechnical Protocols Certificate of Analysis |
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