Anti-Mouse M-CSF (Clone 131621)

Purified Recombinant Mouse M-CSF (>98%)

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Next Day Ambient

Neutralization: This antibody is useful for neutralization of Mouse M-CSF bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Mouse M-CSF (at 2.5 ng/ml) is 4 - 16 µg/ml.

Rat Anti-Mouse macrophage colony stimulating factor (M-CSF) (Clone 131621) recognizes an epitope on Mouse M-CSF. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Macrophage colony-stimulating factor, or M-CSF, is a secreted cytokine which influences hemopoietic stem cells to differentiate into macrophages or other related cell types. M-CSF is a key regulator of cellular proliferation, differentiation, and survival of blood monocytes, tissue macrophages and their progenitor cells. It enhances cytotoxicity, superoxide production, phagocytosis, chemotaxis, and secondary cytokine production in monocytes and macrophages.¹ It binds to the Colony stimulating factor 1 receptor. M-CSF is known as one of the factors essential for osteoclast development.²

1. Nemunaitis, J. et al. (1998) Clin. Infect. Dis. 26: 1279
2. Ko, JS. et al. (2002) Exp Mol Med 34: 340