Anti-Mouse M-CSF – Biotin
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Goat Immunogen Purified Recombinant Mouse M-CSF (Accession # Q3U4F9) Formulation This biotinylated polyclonal antibody has been 0.2 µm filtered and lyophilized from modified 1X PBS, pH 7.2 – 7.4 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831228 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Mouse M-CSF this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Mouse M-CSF is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:M1098. ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 0.1-0.4 µg/ml when used in conjunction with PN:M1276 as the capture antibody at 2-8 µg/ml and an optimal second step reagent such as PN:A106 for the detection of Mouse M-CSF. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Goat Anti-Mouse Macrophage Colony Stimulating Factor (M-CSF) recognizes Mouse Macrophage Colony Stimulating Factor . This antigen affinity purified polyclonal antibody was purified using immobilized antigen agarose chromotography. Background Macrophage colony-stimulating factor, or M-CSF, is a secreted cytokine which influences hemopoietic stem cells to differentiate into macrophages or other related cell types. M-CSF is a key regulator of cellular proliferation, differentiation, and survival of blood monocytes, tissue macrophages and their progenitor cells. It enhances cytotoxicity, superoxide production, phagocytosis, chemotaxis, and secondary cytokine production in monocytes and macrophages.1 It binds to the Colony stimulating factor 1 receptor. M-CSF is known as one of the factors essential for osteoclast development.2 PubMed NCBI Gene Bank ID UniProt.org References & Citations1. Nemunaitis, J. et al. (1998) Clin. Infect. Dis. 26: 1279 2. Ko, JS. et al. (2002) Exp Mol Med 34: 340 Technical ProtocolsELISA Det  Certificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
