Anti-Mouse MCP-1
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Goat Immunogen Purified Recombinant Mouse JE/MCP-1 (>98%) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831063 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Mouse MCP-1 this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Mouse MCP-1 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:J100. ELISA Capture: Suitable for use at concentration of 0.2-0.8 µg/mL. Use in combination with: Detection Reagent: Mouse CCL2/JE/MCP‑1 Biotinylated Antibody. Standard: Recombinant Mouse CCL2/JE/MCP-1 Protein. Additional Applications Reported In Literature ? Neutralization: Measured by its ability to neutralize CCL2/JE/MCP‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR2A. The Neutralization Dose (ND50) is typically 0.5-2.5 µg/mL in the presence of 0.04 µg/mL Recombinant Mouse CCL2/JE/MCP‑1. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Rabbit Anti-Mouse Monocyte Chemotactic Protein (JE/MCP-1) recognizes Mouse JE/MCP-1. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Monocyte chemotactic protein-1 (MCP-1) is an essential chemokine produced predominantly by macrophages and endothelial cells and is a potent chemotactic factor for monocytes.1,2,3 It can induce the proliferation and activation of killer cells known as CHAK (CC-Chemokine-activated killer). MCP-1 is also a potent activator of human basophils, induces degranulation and the release of histamines. MCP-1 is involved in the pathogenesis of atherosclerosis and myocardial infarction.4 PubMed NCBI Gene Bank ID UniProt.org Research Area Other Molecules References & Citations1. Yoshimura, T. et al. (1989) J. Exp. Med. 169: 1449 2. Oppenheim, JJ. et al. (1989) J. Exp. Med. 169: 1485 3. Rollins, BJ. et al. (1997) Blood 90: 909 4. Benjamin, EJ. et al. (2005) Ciculation 112: 1113 Technical Protocols N  Certificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
