Anti-Mouse MHC Class I (H-2Kk, H-2Dk) (Clone 15-3-1S) – Biotin

C3H spleen cells

This Biotinylated antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

Liquid

This biotinylated antibody is stable when stored at 2-8°C. Do not freeze.

Research Use Only

2 – 8° C Wet Ice

15-3-1S activity is directed against MHC Class I H-2Kk and H-2Dk. 15-3-1S cross reacts with the H-2r haplotype.

H-2, the murine major histocompatibility complex (MHC), is composed of a diverse group of antigens divided into class I and II proteins that function in immune response¹. MHC class I molecules bind peptides generated by the degradation of cytosolic proteins, and then display those peptides on the cell surface. Generally, these peptides are derived from normal metabolism, but they can also be derived from foreign proteins during viral infection or allotransplantation. When peptides are recognized as foreign, cytotoxic T lymphocytes specific to the MHC class I-peptide complex kill the presenting cell. H-2Kk and H-2Dk are MHC class I proteins originally identified in the C3H mouse².

15-3-1S was generated by immunizing C3H mice with spleen cells from C3H mice³. Subsequently, spleen cells from the immunized mice were fused with Sp2/0-Ag14 myeloma cells to create hybridoma lines. 15-3-1S was characterized by performing cytotoxic titer of hybridoma ascites on a panel of H-2 haplotypes⁴. Additionally, blocking ability and specificity were analyzed. 15-3-1S has relatively low affinity for H-2Kk according to radioactive binding assays with B10.A spleen cells.

H-2Kk is present on 100% of T cells, B cells, erythrocytes, macrophages, cells of endocrine origin including B cells of Langerhans as well as endothelial and epithelial tissues. H-2Dk is expressed on nucleated cells from mice of the H-2Dk haplotype.

CD3/TCR, CD8

1 Yoshida R. Adv Immunol. 124:207-247. 2014.
2 Watts S, Vogel JM, Harriman WD, et al. J Immunol. 139(11):3878-3885. 1987.
3 Ozato K, Mayer N, Sachs DH. J Immunol. 124(2):533-540. 1980.
4 Epstein SL, Ozato K, Sachs DH. J Immunol. 125(1):129-135. 1980.
5 Lenz A, Heufler C, Rammensee HG, et al. Proc Natl Acad Sci U S A. 86(19):7527-7531. 1989.
6 Caughman SW, Sharrow SO, Shimada S, et al. Proc Natl Acad Sci U S A. 83(19):7438-7442.1986.
7 Madsen JC, Superina RA, Wood KJ, et al. Nature. 332(6160):161-164. 1988.