Anti-Mouse MHC class II (I-A) (Clone Y-3P) – Biotin

BALB/c x C57BL/6 F1 mouse spleen cells

This Biotinylated antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

Liquid

This biotinylated antibody is stable when stored at 2-8°C. Do not freeze.

Research Use Only

2 – 8° C Wet Ice

Y-3P activity is directed against mouse MHC class II (I-A) glycoprotein antigens, including the haplotypes I-Ab, I-Af, I-Ap, I-Aq, I-Ar, I-As, I-Au, I-Av, and weakly I-Ak. Y-3P also reacts with the equivalent complexes in rats.

H-2, the murine major histocompatibility complex (MHC), is composed of a diverse group of antigens divided into class I and II proteins that function in immune response¹. Class II molecules, also known as Ia antigens, regulate recognition of foreign antigens on the surfaces of antigen presenting cells and play a major role in the mixed lymphocyte response². Mice have two class II isotypes, I-A and I-E, each of which is a glycoprotein composed of an ⍺ and β subunit. The N-terminal α1 and β1 domains of the MHC class II isotype form the antigen- binding groove, which binds 13-25 aa peptides derived from exogenous antigens³.

On APCs, MHC class II molecules play a critical role in the adaptive immune response by presenting phagocytosed antigens to helper CD4 T cells. The T cell receptor (TCR)/CD3 complex of CD4 T cells interacts with peptide-MHC class II, which induces CD4 T cell activation leading to the coordination and regulation of other effector cells. CD4 molecules also bind to MHC class II, which helps augment TCR signaling⁴. Additionally, MHC class II expressed on activated T cells are capable of antigen presentation⁵ and can transduce signals into T cells, enhancing T cell proliferation and activity⁶.

Y-3P was generated by repeatedly immunizing primed mice with activated T cells over the course of a year⁷. Y-3P reacts with I-A subregion-controlled A ⍺: A β complexes of all mouse strains except the responder strain H-2d. Y-3P is commonly used for in vivo blockade of TCR stimulation^8,9 and MHC class II blocking^{10,11,12,13,14,15}.

MHC class II molecules are constitutively expressed on professional antigen-presenting cells (APCs), including macrophages, monocytes, dendritic cells (DCs), and B cells, and are induced on T cells upon activation.

CD3/TCR, CD4

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14. Kawabe T, Yi J, Kawajiri A, et al. Nat Commun. 11(1):3366. 2020.
15. Kruse B, Buzzai AC, Shridhar N, et al. Nature. 618(7967):1033-1040. 2023.
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