Anti-Mouse NK1.1 Antibody – Purified in vivo GOLD™ Functional Grade
Anti-Mouse NK1.1 Antibody – Purified in vivo GOLD™ Functional Grade
Product No.: N123
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Clone PK136 Target NK1.1 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Natural Killer Cells, KR-P1C, NKR-P1B, Ly-55, CD161b, CD161c Isotype Mouse IgG2a k Applications B , CyTOF® , Depletion , FC , in vivo , IP , WB |
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Mouse Recommended Isotype Controls Recommended Isotype Controls Recommended Dilution Buffer Immunogen NK-1+ cells from mouse spleen and bone marrow Product Concentration ≥ 5.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal NK1.1 antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2737553 Applications and Recommended Usage? Quality Tested by Leinco Flow Cytometry: The suggested use of this NK1.1 antibody is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. Additional Applications Reported In Literature ? CyTOF® (Validated) IP Depletion Block IHC IF (Reported in the literature) Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity This NK1.1 antibody (clone PK136) recognizes mouse NK1.1. Background NK1.1 is a type II membrane protein that is part of the C-type lectin superfamily. NK1.1 contains a transmembrane domain and a cytoplasmic domain that are characteristic of C-type lectins. The function of NK cells is to mediate cytotoxicity and to secrete cytokines after immune stimulation. NK1.1 has been correlated with lysis of tumor cells In vitro and rejection of bone marrow allografts In vivo. It is also involved in NK cell activation, IFN-γ production, and cytotoxic granule release. NK-1.1 is commonly used as a mouse NK cell marker. Antigen Distribution NK-1.1 is encoded by the NKR-P1B/NKR-P1C gene and expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129. Function NK cell activation, IFN-γ production, cytotoxic granule release PubMed NCBI Gene Bank ID UniProt.org Research Area Immunology . Innate Immunity References & Citations1.) Ardolino, M. et al. (2018) J Clin Invest. 128(10):4654-4668. PubMed 2.) Koo, GC. and Peppard, JR. (1986) Hybridoma 3:301 3.) Raju et al. (2019) Cell Reports. 29:2556–2564 Journal Link Technical ProtocolsB CyTOF® Depletion     Certificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
