Anti-Mouse RANK
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Mouse Immunogen Sf 21-Derived Recombinant Mouse RANK Extracellular Domain Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831694 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Mouse RANK this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Mouse RANK is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Additional Applications Reported In Literature ? IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 10 µg/ml. The recommended secondary antibody for IHC is PN:M114. For chromogenic detection with high signal and low background use PN:D100 or PN:K107. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Mouse Anti-Mouse Receptor Activator of NF-κB (RANK) recognizes Mouse RANK. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background RANK (Receptor Activator of Nuclear Factor κ B), also known as TRANCE Receptor is a type I membrane protein which is TNF receptor family member1 expressed on the surface of osteoclasts and is involved in the activation of osteoclasts upon ligand binding. It is also expressed on dendritic cells and facilitates immune signaling. RANK is highly expressed in mature osteoclasts.2 RANK is the intrinsic cell surface determinant that mediates osteoprotegerin ligand effects on bone resorption and remodeling as well as the physiological and pathological effects of calciotropic hormones and proresorptive cytokines.3 PubMed NCBI Gene Bank ID UniProt.org References & Citations1. Aggarwal, BB. et al. (1998) J Biol Chem. 273:20551 2. Nicholson, GC. et al. (1999) FEBS Lett. 463:295 3. Boyle, WJ. et al. (2000) Proc Natl Acad Sci USA 97:1566 Technical ProtocolsIHC FFPE  Certificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
