Anti-Mouse RANTES – Biotin
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Goat Immunogen Purified Recombinant Mouse RANTES (>98%) Formulation This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling This lyophilized biotinylated polyclonal antibody should be stored desiccated at -20°C. The reconstituted conjugate is stable for at least one month when stored at 2 - 8°C. For long term storage aseptically aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid More Than One Freeze Thaw Cycle. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831729 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Mouse RANTES this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Mouse RANTES is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:R135. ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 0.1-0.4 µg/ml when used in conjunction with PN: as the capture antibody at µg/ml and an optimal second step reagent such as PN:A106 for the detection of Mouse RANTES. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Goat Anti-Mouse Regulation Upon Activation Normal T Cell Express Sequence (RANTES) recognizes Mouse RANTES. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background RANTES also known as Chemokine (C-C motif) ligand 5 (CCL5), a CC-chemokine1 is an important mediators of the immune and inflammatory response.2 It is also a potent chemoattractant for T cells 3 and eosinophils.4 RANTES is involved in many inflammatory diseases, for example, bronchial asthma, delayed-type hypersensitivity reactions, viral infections, arthritis, chronic eosinophilic pneumonia, and idiopathic interstitial pneumonia.5 PubMed NCBI Gene Bank ID References & Citations1. Lee, SC. et al. (2004) J Neurochem. 90: 297 2. Borkowski, J. et al. (2004) Arch Immunol Ther Exp (Warsz) 52: 201 3. Geiger, H. et al. (2005) Kidney Blood Press Res 28: 48 4. Hirata, I. et al.(2009) Dig Dis Sci. 54(6):1247-52. 5. Emilie, D. et al. (1994) J Exp Med. 179: 1689 Technical ProtocolsELISA Det  Certificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
