Anti-Mouse/Rat CD71 – Purified in vivo GOLD™ Functional Grade
Anti-Mouse/Rat CD71 – Purified in vivo GOLD™ Functional Grade
Product No.: C853
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Clone OX-26 Target CD71 Formats AvailableView All Product Type Monoclonal Antibody Isotype Mouse IgG2a Applications FC , IHC , in vivo |
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Antibody DetailsProduct DetailsReactive Species Mouse/Rat Host Species Mouse Recommended Isotype Controls Recommended Dilution Buffer Product Concentration ≥ 5.0 mg/ml Endotoxin Level <1.0 EU/µg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.0-7.5, 0.005% pS80 stabilizing buffer, with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco FC IHC Additional Applications Reported In Literature ? IHC FC Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone OX-26 reacts with rat CD71 (TfR1). Background CD71 is a transferrin receptor on the cell surface that mediates the uptake of transferrin-iron complexes, thereby mediating cellular proliferation1. CD71 is a homodimeric glycoprotein containing 760 amino acids and it binds to diferric transferrin2. Binding by diferric transfferin causes CD71 to be internalized, and diferric iron is subsequently released3. CD71 is highly expressed on malignant cells, as iron uptake is necessary for aberrant cell proliferation4. Antigen Distribution CD71 is expressed on the surface of proliferating cells, reticulocytes, and erythroid precursors. UniProt.org Research Area Immunology References & Citations1. Aisen P. Int J Biochem Cell Biol. Nov;36(11):2137-43. 2004.
2. Lawrence CM, Ray S, Babyonyshev M, et. al. Science. Oct 22;286(5440):779-82. 1999. 3. Marsee DK, Pinkus GS, Yu H. Am J Clin Pathol. Sep;134(3):429-35. 2010. 4. Candelaria PV, Leoh LS, Penichet ML, Daniels-Wells TR. Front Immunol. Mar 17;12:607692. 2021 5. Jiménez E, Sacedón R, Vicente A, et. al. J Immunol. May 15;168(10):5005-13. 2002. 6. Fabriek BO, Polfliet MM, Vloet RP, et. al. Blood. Jun 15;109(12):5223-9. 2007. Technical Protocols   Certificate of Analysis |
Formats Available
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
