Anti-Mouse TCR β chain – APC
Anti-Mouse TCR β chain – APC
Product No.: T665
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Clone H57-597 Target TCR beta Formats AvailableView All Product Type Monoclonal Antibody Alternate Names TCR-β chain, TCR-β, β-TCR Isotype IgG Applications FC |
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Armenian Hamster Immunogen Affinity purified TCR from mouse DO-11.10 cells Product Concentration 0.2 mg/ml Formulation This Allophycocyanin (APC) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative. Storage and Handling This Allophycocyanin (APC) conjugate is stable when stored at 2-8°C. Do not freeze. Country of Origin USA Shipping Next Day 2-8°C Excitation Laser Red Laser (650 nm) RRIDAB_2832107 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this TCR β chain (clone H57-597) antibody for staining cells in flow cytometry is ≤ 0.25 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone H57-597 recognizes an epitope on the constant region of mouse TCRβ. Clone H57-597 does not cross-react with γδ T cells. Background TCRβ antibody, clone H57-597, recognizes the beta chain of the T cell receptor (TCRβ), expressed on αβ thymocytes and T lymphocytes, as well as natural killer T (NKT) cells1. TCRβ is a transmembrane protein that consists of an amino-terminal variable region, a constant region, and a stalk segment covalently linked to the TCR alpha chain (TCRα) via a disulfide bond2. TCRα/β does not contain signaling domains and is instead noncovalently associated with the CD3 complex (TCR-CD3), essential for TCR signaling3,4. TCRα/β recognizes antigens bound to major histocompatibility complex (MHC) molecules, resulting in phosphorylation of CD3 ITAMs and subsequent T cell activation, proliferation, and survival5,6. Antigen Distribution TCRβ is expressed on αβ thymocytes, peripheral T cells, and NKT cells. Ligand/Receptor Peptide bound-MHC class I and II Function Antigen recognition, T cell activation PubMed NCBI Gene Bank ID UniProt.org References & Citations1. Krijgsman D, et al. (2018) Front Immunol. 9:367 2. Clevers H, et al. (1988) Annu Rev Immunol. 6:629-62 3. Kuhns MS, Badgandi HB. (2012) Immunol Rev. 250(1):120-43 4. Wucherpfennig KW, et al. (2010) Cold Spring Harb Perspect Biol. 2(4):a005140 5. Samelson LE. (2002) Annu Rev Immunol. 20:371-94 6. Borroto A, et al. (2014) Immunol Lett. 161(1):113-7 Technical Protocols Certificate of Analysis |
Formats Available
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Prod No. | Description |
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T663 | |
T668 | |
T669 | |
T670 | |
T671 | |
T664 | |
T900 | |
T662 | |
T648 | |
T666 | |
T665 | |
T772 |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
