Anti-Mouse TCR β chain – DyLight® 488
Anti-Mouse TCR β chain – DyLight® 488
Product No.: T668
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Clone H57-597 Target TCR beta Formats AvailableView All Product Type Monoclonal Antibody Alternate Names TCR-β chain, TCR-β, β-TCR Isotype IgG Applications FC , IHC FF |
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Armenian Hamster Immunogen Affinity purified TCR from mouse DO-11.10 cells Product Concentration 0.2 mg/ml Formulation This DyLight® 488 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative. Storage and Handling This DyLight® 488 conjugate is stable when stored at 2-8°C. Do not freeze. Country of Origin USA Shipping Next Day 2-8°C Excitation Laser Blue Laser (493 nm) RRIDAB_2832110 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this TCR β chain (clone H57-597) antibody for staining cells in flow cytometry is ≤ 0.25 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. Additional Applications Reported In Literature ? IHC FF Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone H57-597 recognizes an epitope on the constant region of mouse TCRβ. Clone H57-597 does not cross-react with γδ T cells. Background TCRβ antibody, clone H57-597, recognizes the beta chain of the T cell receptor (TCRβ), expressed on αβ thymocytes and T lymphocytes, as well as natural killer T (NKT) cells1. TCRβ is a transmembrane protein that consists of an amino-terminal variable region, a constant region, and a stalk segment covalently linked to the TCR alpha chain (TCRα) via a disulfide bond2. TCRα/β does not contain signaling domains and is instead noncovalently associated with the CD3 complex (TCR-CD3), essential for TCR signaling3,4. TCRα/β recognizes antigens bound to major histocompatibility complex (MHC) molecules, resulting in phosphorylation of CD3 ITAMs and subsequent T cell activation, proliferation, and survival5,6. Antigen Distribution TCRβ is expressed on αβ thymocytes, peripheral T cells, and NKT cells. Ligand/Receptor Peptide bound-MHC class I and II Function Antigen recognition, T cell activation PubMed NCBI Gene Bank ID UniProt.org References & Citations1. Krijgsman D, et al. (2018) Front Immunol. 9:367 2. Clevers H, et al. (1988) Annu Rev Immunol. 6:629-62 3. Kuhns MS, Badgandi HB. (2012) Immunol Rev. 250(1):120-43 4. Wucherpfennig KW, et al. (2010) Cold Spring Harb Perspect Biol. 2(4):a005140 5. Samelson LE. (2002) Annu Rev Immunol. 20:371-94 6. Borroto A, et al. (2014) Immunol Lett. 161(1):113-7 Technical Protocols IHC FF Certificate of Analysis |
Formats Available
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Prod No. | Description |
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T663 | |
T668 | |
T669 | |
T670 | |
T671 | |
T664 | |
T900 | |
T662 | |
T648 | |
T666 | |
T665 | |
T772 |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
