Anti-Mouse TIM-2 – FITC

TIM-2-Ig chimera protein consisting of extracellular domain residues 1-230 and the Fc portion of mouse IgG2

This Fluorescein (FITC) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

Liquid

This Fluorescein conjugate is stable when stored at 2-8°C. Do not freeze.

Research Use Only

2 – 8° C Wet Ice

Blue Laser (488 nm)

RMT2-25 activity is directed against mouse TIM-2.

The T cell/transmembrane, immunoglobulin, and mucin (TIM) family plays a critical role in immune response, transplant tolerance, autoimmunity, and allergy and asthma regulation¹. The TIM family consists of eight members, including TIM-2 which is only found in rodent genomes. TIM-2 is likely a gene duplication of TIM-1. TIM-2 is a type I cell surface glycoprotein that has an N-terminal immunoglobulin-like (Ig) domain, a mucin domain with O- and N-linked glycosylations, a single transmembrane domain, and a cytoplasmic region with tyrosine phosphorylation motif. Unlike other TIM family proteins, TIM-2 does not bind PtdSer. TIM-2 is a receptor for H-ferritin. TIM-2 functions as a negative regulator of T cell activation and Th2 responses.

RMT2-25 was generated by immunizing Sprague Dawley rats with a TIM-2-Ig chimera protein consisting of murine TIM-2-Ig (extracellular domain residues 1-230) and the Fc portion of mouse IgG2a² . LN cells were fused with P3U1 myeloma cells. The RMT2-25 hybridoma was selected using flow cytometry, based on reactivity to mouse TIM-2 transfected cells, but not parental cells. RMT2-25 inhibits the binding of H-ferritin to TIM-2. RMT2-25 also significantly enhances B cell proliferation and activation in vitro by agonistic stimulation of TIM-2.

TIM-2 is expressed by activated T cells, particularly after differentiation under Th2 conditions. TIM-2 is not expressed by T cells differentiated under Th1 conditions. TIM-2 is also expressed by splenic and germinal B cells. Other cells expressing TIM-2 include epithelial cells in the liver and kidney as well as oligodendrocytes.

Sema4A, H-ferritin

1 Freeman GJ, Casasnovas JM, Umetsu DT, et al. Immunol Rev. 235(1):172-189. 2010.
2 Kawamoto T, Abe Y, Ito J, et al. Arthritis Res Ther. 13(2):R47. 2011.