Anti-Mouse TIM-2 – Purified in vivo GOLD™ Functional Grade

TIM-2-Ig chimera protein consisting of extracellular domain residues 1-230 and the Fc portion of mouse IgG2

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.

Liquid

Functional grade preclinical antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.

Research Use Only

2 – 8° C Wet Ice

RMT2-25 activity is directed against mouse TIM-2.

The T cell/transmembrane, immunoglobulin, and mucin (TIM) family plays a critical role in immune response, transplant tolerance, autoimmunity, and allergy and asthma regulation¹. The TIM family consists of eight members, including TIM-2 which is only found in rodent genomes. TIM-2 is likely a gene duplication of TIM-1. TIM-2 is a type I cell surface glycoprotein that has an N-terminal immunoglobulin-like (Ig) domain, a mucin domain with O- and N-linked glycosylations, a single transmembrane domain, and a cytoplasmic region with tyrosine phosphorylation motif. Unlike other TIM family proteins, TIM-2 does not bind PtdSer. TIM-2 is a receptor for H-ferritin. TIM-2 functions as a negative regulator of T cell activation and Th2 responses.

RMT2-25 was generated by immunizing Sprague Dawley rats with a TIM-2-Ig chimera protein consisting of murine TIM-2-Ig (extracellular domain residues 1-230) and the Fc portion of mouse IgG2a² . LN cells were fused with P3U1 myeloma cells. The RMT2-25 hybridoma was selected using flow cytometry, based on reactivity to mouse TIM-2 transfected cells, but not parental cells. RMT2-25 inhibits the binding of H-ferritin to TIM-2. RMT2-25 also significantly enhances B cell proliferation and activation in vitro by agonistic stimulation of TIM-2.

TIM-2 is expressed by activated T cells, particularly after differentiation under Th2 conditions. TIM-2 is not expressed by T cells differentiated under Th1 conditions. TIM-2 is also expressed by splenic and germinal B cells. Other cells expressing TIM-2 include epithelial cells in the liver and kidney as well as oligodendrocytes.

Sema4A, H-ferritin

1 Freeman GJ, Casasnovas JM, Umetsu DT, et al. Immunol Rev. 235(1):172-189. 2010.
2 Kawamoto T, Abe Y, Ito J, et al. Arthritis Res Ther. 13(2):R47. 2011.