Anti-Mouse TIM-3 (Clone B8.2C12) – PE

Mouse TIM-3 protein

This R-phycoerythrin (R-PE) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

Liquid

This R-phycoerythrin (R-PE) conjugate is stable when stored at 2-8°C. Do not freeze.

Research Use Only

2 – 8° C Wet Ice

Blue Laser (488 nm) and/or Green Laser (532 nm)/Yellow-Green Laser (561 nm)

B8.2C12 activity is directed against mouse TIM-3 (CD366).

TIM-3 is a member of the T cell immunoglobulin mucin gene family and encodes a type I membrane protein consisting of an immunoglobulin variable-region-like domain, a mucin-like domain, and a tyrosine phosphorylation motif¹. TIM-3 functions as an important immune checkpoint receptor that helps regulate dendritic cell function², T helper type I expansion, and induction of peripheral tolerance³. TIM-3 interacts with GAL-9, PtdSer, HMGB1 and CEACAM1 to activate biochemical pathways such as immune tolerance, T cell depletion, NF-κB signaling, and IL-2 secretion⁴. Additionally, TIM-3 expression correlates with terminal differentiation and exhaustion in tumors as well as chronic infection³. Due to its dysregulation in different types of cancer, TIM-3 blockade is being investigated as an anti-tumor immunotherapy^2,4. TIM-3 also has potential as a prognostic marker in solid tumors⁴.

B8.2C12 was generated by immunizing Lewis and Lou/M female rats (Harlan Sprague-Dawley) with Th1 polarized T cell clones and/or lines, including Th1-specific clone AE7 and in vitro differentiated Th1 cell lines derived from 5B6 and DO11.10 T cell receptor transgenic mice¹. Spleen cells were fused with myeloma cells and a large panel of monoclonal antibodies was screened on Th1 and Th2 cells by flow cytometry. B8.2C12 selectively stained Th1 cells. Gene expression cloning was then used to identify a complementary DNA, which was TIM-3.

TIM-3 (CD366) is expressed on interferon-γ producing T cells, dendritic cells, cytotoxic lymphocytes, exhausted T cells, natural killer cells, Th17, and myeloid cells. TIM-3 is also expressed on CD8 + T cells in the tumor microenvironment as well as intratumoral macrophages and monocytes. TIM-3 is expressed by T helper 1 cells after several rounds of polarization in vitro. TIM-3 is not expressed by naïve T cells.

Expressed on activated Th1 lymphocytes, CD11b+ macrophages, and dendritic cells.

1 Monney L, Sabatos CA, Gaglia JL, et al. Nature. 415(6871):536-541. 2002.
2 Dixon KO, Tabaka M, Schramm MA, et al. Nature. 595(7865):101-106. 2021.
3 Sabatos CA, Chakravarti S, Cha E, et al. Nat Immunol. 4(11):1102-1110. 2003.
4 Sauer N, Janicka N, Szlasa W, et al. Cancer Immunol Immunother. 72(11):3405-3425. 2023.
5 del Rio ML, Cote-Sierra J, Rodriguez-Barbosa JI. Transpl Int. 24(5):501-513. 2011.
6 Cong J, Wang X, Zheng X, et al. Cell Metab. 28(2):243-255.e5. 2018.
7 Chen L, Yang QC, Li YC, et al. Cancer Immunol Res. 8(2):179-191. 2020.
8 Taniguchi H, Caeser R, Chavan SS, et al. Cell Rep. 39(7):110814. 2022.
9 Guo J, De May H, Franco S, et al. Nat Biomed Eng. 6(1):19-31. 2022.