Anti-Mouse CD120a (TNFR1) (Clone 47803)

Product No.: T436


Clone 47803 Target TNF RI Formats AvailableView All Product Type Monoclonal Antibody Alternate Names TNFRSF1A, P55, TBP1, CD120a, FPF, MGC19588, TNF-R, TNF-R55, TNFAR, TNFR1, TNFR55, TNFR60, p55-R, p60, Tumor Necrosis Factor Receptor I Isotype IgG1 Applications ELISA Cap , WB


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Antibody Details Product Details Reactive Species Mouse Host Species Rat Immunogen Purified Recombinant Mouse TNF RI (>98%) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831936 Applications and Recommended Usage? Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:T395. Western Blotting: To detect Mouse TNF RI this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse TNF RI is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:T395. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity Rat Anti-Mouse ttmor Necrosis Factor Receptor I (TNF RI) (Clone 47803) recognizes an epitope on Mouse TNF RI. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background Tumor necrosis factor receptor superfamily, member 1A, (TNFRI) also known as TNFRSF1A or CD120A is the principal receptor through which many of the pro-inflammatory activities of TNF-alpha are mediated.¹ TNFRI contains a death domain through which it interacts with other death-domain proteins to promote cellular responses.² It regulates immune and inflammatory responses.³ PubMed TNF RI References & Citations 1. Dana, R. et al. (2000) Arch Ophthalmol. 118: 1666 2. Donner, DB. et al. (2008) J Immunol. 181: 1288 3. Pasparakis, M. et al. (2008) Nat Immunol. 9: 1015 View product citations for antibody T436 on CiteAb Technical Protocols Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Mouse

Host Species

Rat

Immunogen

Purified Recombinant Mouse TNF RI (>98%)

Endotoxin Level

<0.1 EU/µg as determined by the LAL method

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Storage and Handling

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2831936

Applications and Recommended Usage?
Quality Tested by Leinco

ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:T395.
Western Blotting: To detect Mouse TNF RI this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse TNF RI is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:T395.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Rat Anti-Mouse ttmor Necrosis Factor Receptor I (TNF RI) (Clone 47803) recognizes an epitope on Mouse TNF RI. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Background

Tumor necrosis factor receptor superfamily, member 1A, (TNFRI) also known as TNFRSF1A or CD120A is the principal receptor through which many of the pro-inflammatory activities of TNF-alpha are mediated.¹ TNFRI contains a death domain through which it interacts with other death-domain proteins to promote cellular responses.² It regulates immune and inflammatory responses.³

PubMed

TNF RI

References & Citations

1. Dana, R. et al. (2000) Arch Ophthalmol. 118: 1666
2. Donner, DB. et al. (2008) J Immunol. 181: 1288
3. Pasparakis, M. et al. (2008) Nat Immunol. 9: 1015

View product citations for antibody T436 on CiteAb

Certificate of Analysis

Request COA

Prod No.	Description
T428	Anti-Human CD120a (TNFR1)
T436	Anti-Mouse CD120a (TNFR1) (Clone 47803)
T395	Recombinant Mouse CD120a (TNFR1)
T479	Anti-Human CD120a (TNFR1) – Biotin

Formats Available

Prod No.	Description
T436	Anti-Mouse CD120a (TNFR1) (Clone 47803)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

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Anti-Mouse CD120a (TNFR1) (Clone 47803)