Anti-Rat TRAIL
Anti-Rat TRAIL
Product No.: T543
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Clone 170533 Target TRAIL Formats AvailableView All Product Type Monoclonal Antibody Alternate Names TNFSF10, TL2, TNF-Related Apoptosis-Inducing Ligand Isotype IgG2a Applications ELISA Indirect , WB |
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Antibody DetailsProduct DetailsReactive Species Rat Host Species Mouse Immunogen E. coli-derived recombinant mouse TRAIL/TNFSF10
Pro118-Asn291 Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2832023 Applications and Recommended Usage? Quality Tested by Leinco Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5-1.0 µg/ml with a suitable second step reagent such as PN:M114. The detection sensitivity of this indirect ELISA for Mouse TRAIL is approximately 15 ng/well. Western Blotting: To detect Mouse TRAIL this monoclonal antibody can be used at a concentration of 2-8 µg/mL. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Mouse TRAIL is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:T523. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Mouse Anti-Rat TNF-related apoptosis-inducing ligand (TRAIL) (Clone 170533) recognizes an epitope on Rat TRAIL and does not cross-react with recombinant human (rh) APRIL, rhBAFF, recombinant mouse (rm) BAFF, rhEDA-A2, rmEDA, rhFas Ligand, rmFas Ligand, rhGITR Ligand, rhLIGHT, rhOX40 Ligand, cotton rat TNF-alpha, rhTNF-alpha, rmTNF-alpha, recombinant porcine TNF-alpha, recombinant rat TNF-alpha, recombinant rhesus macaque TNF-alpha, rhTRANCE, rhTWEAK, rhVEGI, or rhTRAIL. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. PubMed NCBI Gene Bank ID UniProt.org References & CitationsTechnical Protocols  Certificate of Analysis |
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Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
