Anti-Mouse TRAIL R2
Anti-Mouse TRAIL R2
Product No.: T474
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Target TRAIL R2 Product Type Polyclonal Antibody Alternate Names TNF-Related Apoptosis-Inducing Ligand Receptor 2, TNFRSF10B, CD262, DR5, KILLER, TRICK2, TRICK2A, TRICK2B, TRICKB, ZTNFR9 Applications ELISA Indirect , FC , N , WB |
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Goat Immunogen NS0-Derived Recombinant Mouse TRAIL Receptor 2 Extracellular Domain Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831974 Applications and Recommended Usage? Quality Tested by Leinco Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing TRAIL R2. A suggested method would be to stain cells expressing TRAIL R2 with 0.25 µg per 1 x 106 cells in a total staining volume of 100 µl followed by PN:G641. Western Blotting: To detect Mouse TRAIL R2 this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T263. The detection limit for Mouse TRAIL R2 is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Additional Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Mouse TRAIL R2 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse TRAIL R2 (at 12 ng/ml) is 0.4 - 1.2 µg/ml. CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Goat Anti-Mouse TNF-Related Apoptosis-Inducing Ligand Receptor 2 (TRAIL R2) recognizes Mouse TRAIL R2. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Tumor necrosis factor-related apoptosis-inducing ligand Receptor 2 (TRAIL-R2) is a cell-surface receptor involved in tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced cell-death signaling.1 The death ligand TRAIL bears high potential as a new anticancer agent, as binding to the death receptors TRAIL-R1 or TRAIL-R2 triggers apoptosis in most cancer cells.2 TRAIL-R2 has been shown to be associated with a decrease in the survival rates of breast cancer patients.3 PubMed NCBI Gene Bank ID UniProt.org References & Citations1. Yoo, NJ. et al. (2001) Oncogene 20: 399 2. Eberle, J. et al. (2005) J Investigative Dermatol. 125: 1010 3. Rimm, D. et al. (2005) Clin Cancer Research 11 Technical Protocols  N  Certificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
