Anti-Mouse TROP-2
- -
- -
Antibody DetailsProduct DetailsReactive Species Mouse Host Species Goat Immunogen NS0-derived mouse TROP-2 extracellular domain Formulation This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831914 Applications and Recommended Usage? Quality Tested by Leinco Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing TROP-2. A suggested method would be to stain cells expressing TROP-2 with 0.25 µg/106 cells in a total staining volume of 200 µl followed by PN:G641. Western Blotting: To detect Mouse TROP-2 this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Mouse TROP-2 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Additional Applications Reported In Literature ? IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 5-15 µg/ml. The recommended secondary antibody for IHC is PN:G505. For chromogenic detection with high signal and low background use PN:D100 or PN:K107. Immunocytochemistry: Suitable for use at concentration of 5-15 µg/mL. CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Goat Anti-Mouse TROP-2 recognizes Mouse TROP-2. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Trop-2 is a cell surface glycoprotein1 that is highly expressed in a wide variety of epithelial cancers. There is little or no expression of Trop-2 in adult somatic tissue.2 Trop-2 is a novel calcium signal transducer3 involved in the regulation of cell- cell adhesion.4 It is an oncogene that has potential as a therapeutic target.2 It is a possible candidate gene for diagnosis and molecular target therapy of colorectal cancer.5 PubMed NCBI Gene Bank ID UniProt.org References & Citations1. Colnaghi, MI. et al. (1992) Hybridoma 11:539 2. Michel, L. et al. (2008) Mol Cancer Ther. 7:280 3. Alberti, S. et al. (1998) Int J Cancer 75:324 4. Piantelli, M. et al. (2007) J Clin Oncol. 25:10510 5. Mori, M. et al. (2006) clin Cancer Res. 12:3057 Technical ProtocolsCyTOF®  ICC IHC FFPE  Certificate of Analysis |
Related Products
- -
- -
Prod No. | Description |
|---|---|
T454 | |
T424 | |
T473 | |
T412 | |
t478 | |
T404 | |
T499 |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
