Anti-Mouse TWEAK R

Anti-Mouse TWEAK R

Product No.: T536


Target TWEAK R Product Type Polyclonal Antibody Alternate Names TNF-related Weak Inducer of Apoptosis Receptor, TNFSF12, DR3LG, Apo3-Ligand Applications CyTOF® , ELISA Cap , FC , IHC , WB


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Antibody Details Product Details Reactive Species Mouse Host Species Goat Immunogen Purified Recombinant Mouse TWEAK R (>98%) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2832017 Applications and Recommended Usage? Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 0.8 µg/ml. A suitable detection antibody is PN:639 at a concentration of approximately 400 ng/ml. A suggested standard for this assay is PN:T524. Western Blotting: To detect Mouse TWEAK R this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Mouse TWEAK R is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:T524. Flow Cytometry: Suitable for use at concentration of 2.5 µg/106 cells. Additional Applications Reported In Literature ? IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 5-15 µg/ml. The recommended secondary antibody for IHC is PN:G505. For chromogenic detection with high signal and low background use PN:D100 or PN:K107. CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Specificity Goat Anti-Mouse TNF-related Weak inducer of Apoptosis Receptor (TWEAK R) recognizes Mouse TWEAK R. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. PubMed TWEAK R NCBI Gene Bank ID 27279 UniProt.org More Information on Uniprot.org References & Citations 1. Nakayama, M. et al. (2003) J. Immunol. 170:341 2. Wiley, SR. et al. (2001) Immunity 15:837 3. Feng, SL. et al. (2000) Am. J. Pathol. 156:1253 4. Desplat-Jégo, S. et al. (2005) Clin. Immunol. 117:15 View product citations for antibody PRODUCT_CODE on CiteAb Technical Protocols CyTOF® Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Mouse

Host Species

Goat

Immunogen

Purified Recombinant Mouse TWEAK R (>98%)

Endotoxin Level

<0.1 EU/µg as determined by the LAL method

Formulation

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

State of Matter

Lyophilized

Storage and Handling

The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2832017

Applications and Recommended Usage?
Quality Tested by Leinco

ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 0.8 µg/ml. A suitable detection antibody is PN:639 at a concentration of approximately 400 ng/ml. A suggested standard for this assay is PN:T524.
Western Blotting: To detect Mouse TWEAK R this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Mouse TWEAK R is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:T524.
Flow Cytometry: Suitable for use at concentration of 2.5 µg/106 cells.

Additional Applications Reported In Literature ?

IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 5-15 µg/ml. The recommended secondary antibody for IHC is PN:G505. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.
CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Goat Anti-Mouse TNF-related Weak inducer of Apoptosis Receptor (TWEAK R) recognizes Mouse TWEAK R. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

PubMed

TWEAK R

NCBI Gene Bank ID

27279

UniProt.org

More Information on Uniprot.org

References & Citations

1. Nakayama, M. et al. (2003) J. Immunol. 170:341
2. Wiley, SR. et al. (2001) Immunity 15:837
3. Feng, SL. et al. (2000) Am. J. Pathol. 156:1253
4. Desplat-Jégo, S. et al. (2005) Clin. Immunol. 117:15

View product citations for antibody PRODUCT_CODE on CiteAb

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