Anti-Mouse Vγ 3 (TCR) – Biotin

AKR mouse dendritic epidermal cell clone 7-17

This biotinylated antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

This biotinylated antibody is stable when stored at 2-8°C. Do not freeze.

Next Day Ambient

Anti-Mouse Vγ 3 TCR antibody recognizes an epitope of the variable region (Vγ 3) gene product. Antibody from clone 536 immunoprecipitates the TCR heterodimer with two subunits of apparent molecular mass of 52 and 42 kDa.

The T cell receptor or TCR is a molecule found on the surface of T lymphocytes that is responsible for recognizing antigens bound to major histocompatibility complex (MHC) molecules. It is a heterodimer consisting of an α and β chain in 95% of T cells, while 5% of T cells have TCRs consisting of γ and δ chains. Engagement of the TCR with antigen and MHC results in activation of its T lymphocyte through a series of biochemical events mediated by associated enzymes, co-receptors and specialized accessory molecules.

The antibody 536 stained less than 1% of cells in adult thymus (AKR), splenocytes, lymph node, adult CD4-8- thymocytes, and adult CD4-8-, T splenocyte. 92% of day 14 old BALB/C fetal thymus, 86% of C57BL/6 dEC (Thy-1+) and 94% of AKR dEC (Thy-1+) stained positive using this antibody.

Havran, et al. (1989) Pro. Natl. Acad. Sci. USA 86:4185