Anti-Mouse VCAM-1 (Clone 112702)
Anti-Mouse VCAM-1 (Clone 112702)
Product No.: V145
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Clone 112702 Target VCAM-1 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Vascular Cell Adhesion Molecule 1, CD106, INCAM-100, VLA4 Isotype IgG1 Applications WB |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Rat Immunogen NS0-Derived Recombinant Mouse VCAM-1 Extracellular Domain Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2832149 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human VCAM-1 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for rhVCAM-1 is approximately 0.5 ng/lane and 5 ng/lane under non-reducing and reducing conditions, respectively. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Simple Western: Suitable for use at concentration of 100 µg/mL. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Rat Anti-Human Vascular Cell Adhesion Molecule 1 (VCAM-1) (Clone 112702) recognizes an epitope on Human VCAM-1. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background Vascular cell adhesion molecule 1, also known as VCAM1 is an immunoglobulin-like adhesion molecule expressed on activated endothelial cells.1 This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. VCAM-1 promotes the adhesion of lymphocytes, monocytes, eosinophils, and basophils. PubMed NCBI Gene Bank ID UniProt.org References & Citations1. Huo, Y. et al. (2001) J Clin Invest 107: 1209 Technical Protocols Certificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
