Anti-Nitrotyrosine Antibody (56456)
Anti-Nitrotyrosine Antibody (56456)
Product No.: 56456
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Clone 39B6 Target Nitrotyrosine Formats AvailableView All Product Type Monoclonal Isotype Mouse IgG2a Applications ELISA , IHC , IP , WB , AM , FCM , ICC/IF |
Data
Immunohistochemistry analysis using Mouse Anti-Nitrotyrosine Monoclonal Antibody, Clone 39B6 (56456). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Nitrotyrosine Monoclonal Antibody (56456) at 1:25000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Magnification: 40x.
Immunohistochemistry analysis using Mouse Anti-Nitrotyrosine Monoclonal Antibody, Clone 39B6 (56456). Tissue: backskin. Species: Mouse. Fixation: Bouin’s Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Nitrotyrosine Monoclonal Antibody (56456) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Backskin obtained from transgenic mice.
Immunohistochemistry analysis using Mouse Anti-Nitrotyrosine Monoclonal Antibody, Clone 39B6 (56456). Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Nitrotyrosine Monoclonal Antibody (56456) at 1:1000000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Magnification: 40x. With anti-microbial.
Immunohistochemistry analysis using Mouse Anti-Nitrotyrosine Monoclonal Antibody, Clone 39B6 (56456). Tissue: Retinal Injury Model. Species: Mouse. Primary Antibody: Mouse Anti-Nitrotyrosine Monoclonal Antibody (56456) at 1:1000. Secondary Antibody: Alexa Fluor 594 Goat Anti-Mouse (red). Courtesy of: Dr. Rajashekhar Gangaraju, University of Indiana, Department of Ophthalmology, Eugene and Marilyn Glick Eye Institute.
Western Blot analysis of Human A549 cells showing detection of Multiple Bands Nitrotyrosine protein using Mouse Anti-Nitrotyrosine Monoclonal Antibody, Clone 39B6 (56456). Lane 1: MW ladder. Lane 2: Human A549 Cells 15 ug). Load: 15 ug. Block: 5% Skim Milk Powder in TBST. Primary Antibody: Mouse Anti-Nitrotyrosine Monoclonal Antibody (56456) at 1:1000 for 2.5 hours at RT with shaking . Secondary Antibody: Goat anti-mouse IgG:HRP at 1:1000 for 1 hour at RT with shaking . Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: Multiple Bands.
Immunohistochemistry analysis using Mouse Anti-Nitrotyrosine Monoclonal Antibody, Clone 39B6 (56456). Tissue: liver tissue . Species: Rat. Primary Antibody: Mouse Anti-Nitrotyrosine Monoclonal Antibody (56456) at 1:1000. Secondary Antibody: FITC Goat Anti-Mouse (green). - -
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Antibody DetailsProduct DetailsReactive Species Canine ⋅ Human ⋅ Mouse ⋅ Rat Host Species Mouse Immunogen 3-(4-hydroxy-3-nitrophenyl-acetamido) propionic acid conjugated to BSA. Product Concentration Lot Specific Formulation PBS, pH 7.4, 50% glycerol, and 0.09% sodium azide. State of Matter Liquid Product Preparation Purified by Protein G affinity chromatography Storage and Handling This antibody is stable for at least one (1) year at -20°C. Avoid repeated freezing and thawing. Regulatory Status For in vitro investigational use only. Not intended for use in therapeutic or diagnostic procedures. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at 1.0ug/mL.
Positive control: Linsidomine (SIN-1)-treated BSA. Immunohistochemistry: use at 1-5ug/mL. These are recommended concentrations. Endusers should determine optimal concentrations for their applications. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity This antibody recognizes 3-nitrotyrosine moieties, species-independent. No
detectable reactivity with non-nitrated tyrosine. Background Nitrotyrosine is a marker for inflammation and nitric oxide (NO) production and is formed in the presence of the active metabolite NO. Most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase, myoglobin, the cytochrome P- 450s, superoxide dismutase, and prostacyclin synthase. Because nitrotyrosine is a stable product of multiple pathways, its plasma concentration may be a useful determinant of NO- dependent damage in vivo. Nitrotyrosine has been detected in inflammatory diseases such as septic shock, rheumatoid arthritis, celiac disease, atherosclerosis, and chronic renal failure. Research Area Neuroscience References & CitationsTechnical Protocols    AM FCM ICC/IF Certificate of Analysis |
Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
