Anti-p84 Antibody (56248)

Product No.: 56248


Clone 5.00E+10 Target p84 Formats AvailableView All Product Type Monoclonal Alternate Names Tho1, Nuclear matrix protein p84, p84N5, hTREX84 Isotype Mouse IgG2b Applications ICC , IF , IHC , IP , WB , Chromatin IP (ChIP) , IHC-P


Select Product Size 100 µg — $587.00	Qty Max: Min: 1 Step: 1 $587.00 ADD TO CART Login For mAb Advantage Pricing Contact Us for Bulk Quantities

Data

Nuclear Matrix Protein p84 antibody [5E10] detects Nuclear Matrix Protein p84 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Nuclear Matrix Protein p84 stained by Nuclear Matrix Protein p84 antibody [5E10] (56248) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

$Detection of human p84/N5 in nuclear fraction by anti-p84/N5 5E10 monoclonal antibody (56248) in western blot experiment. Lane 1: total lysate, Lane 2: cytoplasmic fraction, Lane 3: membrane fraction, Lane 4: nuclear fraction.$ Detection of human p84/N5 in nuclear fraction by anti-p84/N5 5E10 monoclonal antibody (56248) in western blot experiment. Lane 1: total lysate, Lane 2: cytoplasmic fraction, Lane 3: membrane fraction, Lane 4: nuclear fraction.

Nuclear Matrix Protein p84 antibody [5E10] detects Nuclear Matrix Protein p84 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Nuclear Matrix Protein p84 stained by Nuclear Matrix Protein p84 antibody [5E10] (56248) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100.

p84 antibody [5E10] immunoprecipitates p84 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 ug whole cell lysate/extract of p84 protein expressing HepG2 cells B : Control with 3 ug of pre-immune mouse IgG C : Immunoprecipitation of p84 by 3 ug of p84 antibody [5E10] (56248) 7.5% SDS-PAGE The immunoprecipitated p84 protein was detected by p84 antibody [5E10] (56248) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.

Sample (30 ug of whole cell lysate) A: HeLa B: HeLa nucleus 7.5% SDS PAGE 56248 diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Sample (50 ug of whole cell lysate) A: mouse Cerebellum 7.5% SDS PAGE 56248 diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Sample (whole cell lysate) A: 293T 20ug B: 293T 10ug C: 293T 5ug 7.5% SDS PAGE 56248 diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Nuclear Matrix Protein p84 antibody [5E10] detects Nuclear Matrix Protein p84 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. Nuclear Matrix Protein p84 stained by Nuclear Matrix Protein p84 antibody [5E10] (56248) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Nuclear Matrix Protein p84 antibody [5E10] (56248) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.


Antibody Details Product Details Reactive Species Human Host Species Mouse Immunogen Fusion protein containing amino acids 15-374 of human p84 expressed in E. coli. Product Concentration Lot Specific Formulation PBS, pH 7.4. State of Matter Liquid Product Preparation Purified by Protein G affinity chromatography Storage and Handling This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze- thaw cycles. Regulatory Status Research Use Only Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting, immunoprecipitation, and Immunofluorescence: use at 0.5-2 ug/mL.In immunoblots, a band of 84 kD is detected. Positive controls: Lysates of Molt 4, HeLa, or Raji cells. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Specificity This antibody recognizes an 84 kD nuclear matrix protein that was first discovered through its association with the N-terminal region of retinoblastoma protein. Function Required for efficient export of polyadenylated RNA. Acts as component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. Regulates transcriptional elongation of a subset of genes. Involved in genome stability by preventing co-transcriptional R-loop formation.; Participates in an apoptotic pathway which is characterized by activation of caspase-6, increases in the expression of BAK1 and BCL2L1 and activation of NF-kappa-B. This pathway does not require p53/TP53, nor does the presence of p53/TP53 affect the efficiency of cell killing. Activates a G2/M cell cycle checkpoint prior to the onset of apoptosis. Apoptosis is inhibited by association with RB1. NCBI Gene Bank ID THOC1 UniProt.org Q96FV9 Research Area Cancer Research References & Citations View product citations for antibody 56248 on CiteAb Technical Protocols ICC IF Chromatin IP (ChIP) IHC-P Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Human

Host Species

Mouse

Immunogen

Fusion protein containing amino acids 15-374 of human p84 expressed in E. coli.

Product Concentration

Lot Specific

Formulation

PBS, pH 7.4.

State of Matter

Liquid

Product Preparation

Purified by Protein G affinity chromatography

Storage and Handling

This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze- thaw cycles.

Regulatory Status

Research Use Only

Country of Origin

USA

Shipping

Next Day 2-8°C

Applications and Recommended Usage?
Quality Tested by Leinco

Immunoblotting, immunoprecipitation, and

Immunofluorescence: use at 0.5-2 ug/mL.In immunoblots, a band of 84 kD is detected.

Positive controls: Lysates of Molt 4, HeLa, or Raji cells.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

This antibody recognizes an 84 kD nuclear matrix protein that was first discovered through its association with the N-terminal region of retinoblastoma protein.

Function

Required for efficient export of polyadenylated RNA. Acts as component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. Regulates transcriptional elongation of a subset of genes. Involved in genome stability by preventing co-transcriptional R-loop formation.; Participates in an apoptotic pathway which is characterized by activation of caspase-6, increases in the expression of BAK1 and BCL2L1 and activation of NF-kappa-B. This pathway does not require p53/TP53, nor does the presence of p53/TP53 affect the efficiency of cell killing. Activates a G2/M cell cycle checkpoint prior to the onset of apoptosis. Apoptosis is inhibited by association with RB1.

NCBI Gene Bank ID

THOC1

UniProt.org

Q96FV9

Research Area

Cancer Research