Anti-PCNA – FITC

Rat PCNA made in the protein A vector pR1T2T

This Fluorescein (FITC) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

This Fluorescein conjugate is stable when stored at 2-8°C. Do not freeze.

Next Day Ambient

Blue Laser (490 nm)

Clone PC10 recognizes an epitope on mouse PCNA.

PCNA antibody, clone PC10, recognizes proliferating cell nuclear antigen (PCNA), also known as cyclin, a 36 kDa nuclear protein belonging to the DNA sliding clamp family. PCNA is a ring-shaped homotrimer that encircles and slides along DNA^1,2. PCNA is an auxiliary protein of DNA polymerase δ and ε and tethers the polymerase catalytic unit to the DNA template for efficient DNA synthesis^3-6. PCNA also contributes to chromatin remodeling, DNA repair, sister chromatid cohesion, and cell cycle control⁷. Expression levels of PCNA are associated with proliferation and transformation, and PCNA is commonly used as a marker for proliferating cells^8,9. Antibodies to PCNA are detected in sera from patients with systemic lupus erythematosus (SLE)¹⁰. In addition, PCNA is a reliable diagnostic and prognostic biomarker for cancer, as tumor cells express high levels of PCNA¹¹.

PCNA is a ubiquitous nuclear protein present in all cell cycle stages, with the highest expression in the early S phase. Anti-PCNA staining is almost entirely confined to the nucleus and may show a diffuse or granular pattern or a mixture of both.

Ubiquitination, Sumoylation

Increases DNA polymerase δ processibility during elongation of the leading strand.

1. Krishna TS, et al. (1994) Cell. 79(7):1233-43
2. Schurtenberger P, et al. (1998) J Mol Biol. 275(1):123-32
3. Kelman, Z. (1997) Oncogene. 14, 629–640
4. Tan CK, et al. (1986) J Biol Chem. 261(26):12310-6
5. Bravo R, et al. (1987) Nature. 8;326(6112):515-7
6. Prelich G, et al. (1987) Nature. 8;326(6112):517-20
7. Maga G, Hubscher U. (2003) J Cell Sci. 116(Pt 15):3051-60
8. Bravo R, et al. (1982) Prog Clin Biol Res. 85 Pt A:235-48.
9. Celis JE, et al. (1984) Leuk Res. 8(2):143-57
10. Miyachi K, et al. (1978) J Immunol. 121(6):2228-34.
11. Naryzhny SN, Lee H. (2007) FEBS Lett.581(25):4917-20