Anti-Phosphotyrosine Purified

KLH-conjugated phosphotyrosine

This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

This purified antibody is stable when stored at 2-8°C. Do not freeze.

Next Day 2-8°C

Anti-Phosphotyrosine (Clone PY20) recognizes phosphorylated tyrosine residues in all species tested (human, mouse, rat, dog, chicken, and frog). This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. The PY20 antibody has been shown to be useful for flow cytometry, immunoprecipitation, WB, and IF.

Tyrosine phosphorylation represents a major mechanism for the activation of signal transduction pathways in response to growth factors, hormones and cytokines. There are approximately 100 tyrosine kinases that are both receptor tyrsosine kinase receptors for various growth factors and cytokines. The extent and duration of tyrosine phosphorylation are tightly regulated by the 107 protein tyrosine phosphatases. Phosphorylation can occur on threonine, serine, and tyrosine residues.

Phospho-Specific

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2. Häring, HU. et al. (1998) Diabetologia 41:833-838. Article Link
3. Katada, T. et al. (1996) J Biol Chem. 271(3):1534-7. Article Link
4. Häring, HU. et al. (1997) Diabetologia. 40(11):1358-62. Article Link
5. Häring, HU. et al. (1997) Diabetologia. 40: 863–866. Article Link