Anti-PKD/PKCμ (phospho-Ser738) Antibody (43078)

Product No.: 43078


Target PKD/PKCμ (phospho-Ser738) Product Type Polyclonal Isotype Rabbit Applications IF , WB


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Data

Immunoblotting: Detection of PKD/PKCμ in extracts of MCF cells untreated or treated with PMA.

Immunofluorescence:Detection of PKD/PKCμ in methanol-fixed HeLa cells.


Antibody Details Product Details Reactivity Species Human ⋅ Mouse ⋅ Rat Host Species Rabbit Immunogen Peptide sequence that includes phosphorylation site of Serine 738 (E-K-S(p)-F-R) derived from human PKD/PCKu and conjugated to KLH. Product Concentration 1.0 mg/ml Formulation PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. State of Matter Liquid Product Preparation Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide Storage and Handling This antibody is stable for at least one (1) year at -20°C. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at dilution of 1:500- 1:1,000. A band of ~115kDa is detected. Immunofluorescence: use at dilution of 1:100- 1:200. These are recommended working dilutions. End users should determine optimal dilutions for their applications. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Specificity Rabbit Polyclonal Antibody specific to PKD/PKCμ (phospho-Ser738) Background Protein kinase C (PKC), a family of lipid-activated serine kinases, is involved in multiple functions in the regulation of growth control. The PKC-related isoform PKC mu/PKD has been implicated in mitogenic signal cascades because of the activation of p42/p44 MAPK leading to Elk1-mediated gene transcription, and PKC mu/PKD has been shown to be activated via a PKC-dependent pathway. Antigen Details Research Area Phosphospecific Antibodies References & Citations Technical Protocols IF

Antibody Details

Product Details

Reactivity Species

Human

⋅

Mouse

⋅

Rat

Host Species

Rabbit

Immunogen

Peptide sequence that includes phosphorylation site of Serine 738 (E-K-S(p)-F-R) derived from human PKD/PCKu and conjugated to KLH.

Product Concentration

1.0 mg/ml

Formulation

PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

State of Matter

Liquid

Product Preparation

Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide

Storage and Handling

This antibody is stable for at least one (1) year at -20°C.

Country of Origin

USA

Shipping

Next Day 2-8°C

Applications and Recommended Usage?
Quality Tested by Leinco

Immunoblotting: use at dilution of 1:500- 1:1,000. A band of ~115kDa is detected.

Immunofluorescence: use at dilution of 1:100- 1:200.

These are recommended working dilutions.

End users should determine optimal dilutions for their applications.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Rabbit Polyclonal Antibody specific to PKD/PKCμ (phospho-Ser738)

Background

Protein kinase C (PKC), a family of lipid-activated serine kinases, is involved in multiple functions in the regulation of growth control. The PKC-related isoform PKC mu/PKD has been implicated in mitogenic signal cascades because of the activation of p42/p44 MAPK leading to Elk1-mediated gene transcription, and PKC mu/PKD has been shown to be activated via a PKC-dependent pathway.

Antigen Details

Research Area

Phosphospecific Antibodies

References & Citations

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

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Anti-PKD/PKCμ (phospho-Ser738) Antibody (43078)