Anti-PolyHistidine (AD1.1.10) – Purified No Carrier Protein

A polypeptide containing a polyhistidine tag

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Purified antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

This antibody may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Next Day Ice Packs

AD1.1.10 activity is directed against proteins and peptides containing the H-H-H-H-H-H (6His) motif.

AD1.1.10 recognizes proteins and peptides containing the polyhistidine tag (His-tag). The most common His-tag is the H-H-H-H-H-H (6x-His) motif, but His-tags can comprise up to 10 His residues. His-tags can be inserted into a target gene either by cloning the encoding DNA in frame into a His-tag containing vector or by using extended polymerase chain reaction primers that incorporate the motif. His-tags can be inserted at either the N or C terminal of target proteins. The most suitable location depends on the target protein’s characteristics as well as the downstream experimental methods. A variety of purification and detection methods make use of His-tags. Applications include flow cytometry, ELISA, Western blot, dot blot, peptide array, immunohistochemistry, immunofluorescence, immunoprecipitation, immunocytochemistry, histidine tag (His-tag) insertion for epitope mapping (HisMAP), and chromatin immunoprecipitation (ChIP).

His-tags are less likely than other tags to affect functionality of the fusion protein due to their relatively small size. However, His residues exist in mammalian and insect systems, and therefore there may be some background binding, depending on the experimental conditions and/or the system used.

The 6His motif is present in histidine tags.

1. Herrera NG, Morano NC, Celikgil A, et al. ACS Omega. 6(1):85-102. 2020.
2. Els Conrath K, Lauwereys M, Wyns L, et al. J Biol Chem. 276(10):7346-7350. 2001.
3. Suen JL, Wu CH, Chen YY, et al. Immunology. 103(3):301-309. 2001.
4. Yi KS, Choi JA, Kim P, et al. PLoS One. 15(7):e0236172. 2020.
5. Lam M, Marsters SA, Ashkenazi A, et al. Elife. 9:e52291. 2020.
6. Moreland KT, Hong M, Lu W, et al. PLoS One. 9(4):e95333. 2014.
7. Smith MH, Rodriguez EH, Weissman JS. J Biol Chem. 289(37):25670-25677. 2014.
8. Sasakura Y, Kanda K, Yoshimura-Suzuki T, et al. Anal Chem. 76(22):6521-6527. 2004.
9. Asano T, Takei J, Furusawa Y, et al. Monoclon Antib Immunodiagn Immunother. 40(6):243-249. 2021.
10. Calero-Cuenca FJ, Osorio DS, Carvalho-Marques S, et al. Curr Biol. 31(7):1521-1530.e8. 2021.