Anti-Porcine TNF-α (Clone 103314)
Anti-Porcine TNF-α (Clone 103314)
Product No.: T455
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Clone 103314 Target TNF-α Formats AvailableView All Product Type Monoclonal Antibody Alternate Names TNF-alpha, TNFSF2, Cachectin, Differentiation-Inducing Factor (DIF), Necrosin, Cytotoxin Isotype IgG1 Applications N |
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Antibody DetailsProduct DetailsReactive Species Porcine Host Species Mouse Immunogen E. coli-Derived Recombinant Porcine TNF-α Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831955 Additional Applications Reported In Literature ? Neutralization: Measured by its ability to neutralize TNF‑ alpha -induced cytotoxicity in the PK‑15 porcine kidney epithelial cell line. Matthews, N. and M. L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M. J. et al. (eds): IRL Press. 221; Bertoni et al. (1993) J. Immunol. Meth. 160:267. The Neutralization Dose (ND50) is typically 0.015-0.06 µg/mL in the presence of 0.05 ng/mL Recombinant Porcine TNF‑ alpha and 1 µg/mL actinomycin D. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone 103314 recognizes an epitope on mouse TNF-α. This antibody shows approximately 13% cross-reactivity with rhTNF-α and no cross-reactivity with rmTNF-α, rrTNF-α, rhTNF-β, rhAPRIL, rhFas Ligand, rhGITR Ligand, rhLIGHT, rhTrance and rhVEGF. Background TNF-α is a 17.5 kD protein that mediates inflammation and immunity caused by the invasion of viruses, bacteria, and parasites by initiating a cascade of cytokines that increase vascular permeability, thus bringing macrophages and neutrophils to the site of infection. TNF-α secreted by the macrophage causes the blood to clot which provides containment of the infection. TNF-α binding to surface receptors brings about various biologic activities that include cytolysis and cytostasis of many tumor cell lines In vitro, hemorraghic necrosis of tumors In vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils. Antigen Distribution TNF-α is secreted by macrophages, monocytes, neutrophils, T cells, B cells, NK cells, LAK cells. PubMed NCBI Gene Bank ID UniProt.org References & Citations1. El-Harith, EHA. et al. (2004) Saudi Med J. 25: 135 2. Adolf, GR. et al. (1990) Infec Immun. 58: 3996 |
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Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.