Anti-Rat ALK-7 – Biotin
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Antibody DetailsProduct DetailsReactive Species Rat Host Species Goat Immunogen Purified Recombinant Rat ALK-7 (Accession # NP_620790) Formulation This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2828148 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Rat ALK-7 this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Rat ALK-7 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:A345. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Goat Anti-Rat Activin Receptor-Like Kinase 7 (ALK-7) recognizes Rat ALK-7. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Activin receptor-like kinase (ALK) 7 is a type I serine/threonine kinase receptor of the transforming growth factor (TGF)-beta family of proteins.1 ALK7 is most abundantly expressed in human brain, pancreas and colon.2 It negatively regulates glucose-stimulated insulin release in β-cells3 and plays an important role in regulating the functional plasticity of pancreatic islets.4 ALK7 also has an important role in the regulation of body fat deposition. These has implications for the development of treatments against diabetes and obesity.5 PubMed NCBI Gene Bank ID UniProt.org References & Citations1. Kim, SJ. et al. (2004) J Biol Chem. 279: 28458 2. Ritvos, O. et al. (2001) Cytogenet Cell Genet. 95: 157 3. Ibanez, CF. et al. (2008) Proc Natl Acad Sci USA 105: 7246 4. Ibanez, CF. et al. (2008) Health & Medicine Technical Protocols Certificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
